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Rapid propagation method of elaeagnus pungens regenerated plantlets

A fast technology for regenerating plants, applied in the field of plants, to achieve the effects of high survival rate, large-scale planting and large yield

Inactive Publication Date: 2015-01-07
NANJING TONGZE AGRI SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In my country, it is distributed in Jiangsu, Zhejiang, Anhui, Jiangxi, Fujian, Hunan, Hubei, Sichuan, Guizhou, Shaanxi, Yunnan and other places. The main chemical components of the plants of the genus Scorpio are volatile oils, terpenes, alkaloids, flavonoids, etc.; pharmacology The main activities are hypoglycemic, hypolipidemic, anti-lipid oxidation, anti-inflammatory and analgesic, immune, etc. Propagation method: commonly used sowing and cutting propagation, the seed germination rate is only about 50%, tissue culture method is used for the propagation of S. Not yet studied

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] Take the young leaves of Cinnamon sativa, soak them in bleaching powder for 2.5 minutes, wash them with running water for 30 minutes, treat them with hydrogen peroxide on the ultra-clean workbench for 12 minutes, rinse them with sterile water 5 times, and put the sterilized leaves of Cinnamon Cinnamon into the medium formula Carry out callus induction culture in S+2, 4-D2mg / L+KT1mg / l+6-BA1.0mg / L induction medium, add sucrose 30g / L, agar 6g / L, pH5.82, light 1200lx , light time 4h, temperature 22±2°C, the induced callus was inserted into medium S+NAA0.1mg / L+CH300mg / l+6-BA0.5mg / L for callus differentiation induction, adding sucrose 60g / L, agar 6g / L, PH5.82, light 2000lx, light time 10h, temperature 22±2°C, put the sprouts induced and differentiated into rooting medium MS+IAA0.01mg / L+rooting powder, add 20g of sucrose / L, agar 6g / L, PH5.82, light 8000lx, light time 17h, temperature 25±2°C, the height of the test-tube seedlings after 30 days of rooting culture is about 3cm, ...

Embodiment 2

[0011] Take the young leaves of Cinnamon sativa, soak them in bleaching powder for 2.5 minutes, wash them with running water for 30 minutes, treat them with hydrogen peroxide on the ultra-clean workbench for 12 minutes, rinse them with sterile water 5 times, and put the sterilized leaves of Cinnamon Cinnamon into the medium formula Carry out callus induction culture in S+2, 4-D2mg / L+KT1mg / l+6-BA1.0mg / L induction medium, add sucrose 30g / L, agar 6g / L, pH5.82, light 1200lx , light time 4h, temperature 22±2°C, the induced callus was inserted into medium S+NAA0.1mg / L+CH350 mg / l+6-BA1.0mg / L for callus differentiation induction, additional Sucrose 60g / L, agar 6g / L, pH5.82, light 2000lx, light time 10h, temperature 22±2°C, put the sprouts induced and differentiated into rooting medium MS+IAA0.02mg / L+rooting powder, add sucrose 20g / L, agar 6g / L, PH5.82, light 8000lx, light time 17h, temperature 25±2°C, the height of the test-tube seedlings after 30 days of rooting culture is about 3cm,...

Embodiment 3

[0013] Take the young leaves of Cinnamon sativa, soak them in bleaching powder for 2.5 minutes, wash them with running water for 30 minutes, treat them with hydrogen peroxide on the ultra-clean workbench for 12 minutes, rinse them with sterile water 5 times, and put the sterilized leaves of Cinnamon Cinnamon into the medium formula Carry out callus induction culture in S+2, 4-D2mg / L+KT1mg / l+6-BA1.0mg / L induction medium, add sucrose 30g / L, agar 6g / L, pH5.82, light 1200lx , light time 4h, temperature 22±2°C, the induced callus was inserted into medium S+NAA0.1mg / L+CH350 mg / l+6-BA1.0mg / L for callus differentiation induction, additional Sucrose 60g / L, agar 6g / L, pH5.82, light 2000lx, light time 10h, temperature 22±2°C, put the sprouts induced and differentiated into rooting medium MS+IAA0.01mg / L+rooting powder, add sucrose 20g / L, agar 6g / L, PH5.82, light 8000lx, light time 17h, temperature 25±2°C, the height of the test-tube seedlings after 30 days of rooting culture is about 3cm,...

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Abstract

The invention provides a rapid propagation method of elaeagnus pungens regenerated plantlets. The rapid propagation method comprises the steps of obtaining of aseptic leaves, induction of calluses, differentiation of calluses, rooting culture, acclimatization and transplanting, and the like. The elaeagnus pungens prepared by the rapid propagation method is good in reproductive rate, high in regeneration rate and short in growth cycle; as a result, a large quantity of elaeagnus pungens seedlings can be obtained in short time.

Description

technical field [0001] The invention relates to a rapid propagation method for the tissue culture of pelagics seeds, belonging to the technical field of plants. Background technique [0002] Hudaozi Elaeagnus pungens , alias Banchunzi, sweet stick, queer crisp, goat's milk, belongs to the evergreen shrubs of the euphratica family. Fruit is sweet and edible when ripe. The roots, leaves and fruits are used for medicinal purposes. It is mainly distributed in the temperate and subtropical regions of the northern hemisphere. It is native to China. There are 41 species in 2 genera in China. The cold resistance is relatively strong. In the southern part of North China, it can survive the winter in the open field, and can endure the absolute low temperature of about minus 8°C. The optimum temperature for growth is 24-34°C, and it can withstand high temperature and heat. It grows under sparse forests on hillsides and in damp valleys, but it is not afraid of sun exposure and has s...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 杨存
Owner NANJING TONGZE AGRI SCI & TECH
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