Application of azaazulene alkaloids topreparing medicine for preventing or treating pulmonary fibrosis
A technology for pulmonary fibrosis and alkaloids, applied in drug combinations, active ingredients of heterocyclic compounds, organic chemistry, etc., can solve problems such as azazulenes-type alkaloids that have not yet been retrieved, and reduce the degree of pulmonary fibrosis with low price , the effect that the material is easy to obtain
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0068] Preparation and structure identification of embodiment 1 alkaloid
[0069] 1. Medicinal materials and reagents
[0070] The medicinal material is the dried root of Stemonasessilifolia (Miq.) Miq., which was purchased from Bozhou Medicinal Materials Market in Anhui. Reagents such as ethanol, dichloromethane, and methanol were all analytically pure.
[0071] 2. Extraction and separation
[0072] 3 times of reflux extraction with 95% ethanol, concentrated under reduced pressure until there is no alcohol smell, acidified with 5% dilute hydrochloric acid to pH 1-2, filtered, the filtrate was alkalized with concentrated ammonia water to pH 10, extracted with chloroform, recovered chloroform to obtain the total biological base site. The total alkaloids were eluted by silica gel column chromatography, dichloromethane-methanol gradient (100:0~100:5), and the same fractions were combined according to the TLC results, and then subjected to repeated silica gel column chromatogra...
Embodiment 2
[0082] The preparation of embodiment 2 pulmonary fibrosis animal model
[0083] 1. Main reagents and experimental animals
[0084] The bleomycin used in the experiment was purchased from Nippon Kayaku Co., Ltd., batch number 730342.
[0085] The SPF grade C57BL / 6 mice (female, 8 weeks old) used in the experiment were purchased from the Comparative Medicine Center of Yangzhou University.
[0086] 2. Model Preparation
[0087] Bleomycin-induced pulmonary fibrosis in mice is an internationally recognized animal model for screening anti-pulmonary fibrosis drugs. Acute lung injury caused by bleomycin modeling, mainly manifested as inflammation at the initial stage, fibrosis began to appear in about 10 days, and a large amount of collagen concentrated in the lungs, these pathological changes are very similar to those of idiopathic pulmonary fibrosis in clinical practice resemblance. Although the pathogenesis and mechanism of idiopathic pulmonary fibrosis are not clear, both bleo...
Embodiment 3
[0089] Example 3 Identification of four alkaloids and Kebiqing anti-pulmonary fibrosis activity in mice
[0090] This example aims to study the anti-pulmonary fibrosis activity of the alkaloids obtained in Example 1 to identify whether they have an anti-pulmonary fibrosis effect. Since these alkaloids are the active components of cough suppressant in Baibu, a traditional Chinese medicine, in order to know whether the cough suppressant can resist pulmonary fibrosis, this example chooses a commonly used cough suppressant in clinical practice——pentoxyverine, pentoxyverine citrate tablet, cough suppressant Positive control drugs commonly used in pharmacological experiments) were simultaneously screened for anti-pulmonary fibrosis activity.
[0091] 1. Main reagents and experimental animals
[0092] Compounds I-IV were obtained according to Example 1, and the purity was >98%. Kebiqing was purchased from Sinopharm Rongsheng Pharmaceutical Co., Ltd., batch number 13110221. The ble...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More