Sterilizing method and sterilizer for small orchidaceae seeds

Abstract

The invention discloses a sterilizing method and a sterilizer for small orchidaceae seeds. By arranging the sterilizer and a relative sterilizing solution and adopting the scientific solution concentration and the sterilizing time, during sterilization, the seeds can be fully and uniformly in contact with the sterilizing solution (the sterilizing solution can uniformly pass through grids in a steel wire net to flow into or out of the sterilizer); meanwhile, during later cultivation, only the steel wire net on a box cover needs to be replaced (namely the mesh number of the steel wire net is reduced and the size of the steel wire net is increased), and the seeds can uniformly pass through the steel wire net to flow into a culture medium so as to finish sterilized cultivation. According to the method, the seeds can be uniformly in contact with a sterilizing agent in the sterilizing process, so that the contact time of the seeds and the sterilizing agent is prolonged; the sterilizing time can be effectively controlled in the sterilizing process; the cracked seeds can be fully utilized, and the seed germination period can be effectively prolonged for non-cracked seeds. Meanwhile, the sterilizer is low in manufacturing cost, convenient to operate, easy to make and reusable.

Description

technical field [0001] The invention relates to a method for sterilizing small orchid seeds and a sterilizer, belonging to the technical field of orchid tissue sterilization. Background technique [0002] Orchidaceae plants are widely used in traditional Chinese medicinal materials, flowers, greening and other fields. Usually the seeds of orchids are very small, such as Baiji, Dendrobium, Rhododendron, Gastrodia elata, etc., and their shapes can only be clearly seen under a microscope. Most orchids have no endosperm, only a few embryos that are not fully developed, resulting in such as white and , Dendrobium, Rhododendron and other seeds are difficult to germinate in the natural environment. Under sterile conditions, using tissue culture for artificial sexual reproduction is one of the effective means for the rapid propagation of most orchids, and in the process of tissue culture, the aseptic treatment of seeds, especially the aseptic treatment of dehiscated capsules, has b...

AI Technical Summary

Problems solved by technology

Under sterile conditions, using tissue culture for artificial sexual reproduction is one of the effective means for the rapid propagation of most orchids, and in the process of tissue culture, the aseptic treatment of seeds, especially the aseptic treatment of dehiscated capsules, has become a key technology One; for the aseptic treatment of small cracked seeds such as white and dendrobium, usually use gauze to wrap the seeds for treatment, but in this process, there will be uneven sterilization, insufficient, adhesion, difficult sowing, Easy to pollute and other phenomena, resulting in waste of resources

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