Tissue culture propagation method for rhododendron hancockii

A technology of Rhododendron in southern Yunnan and tissue culture, applied in the field of plant tissue culture, can solve the problems of difficulty in introduction and domestication, natural scarcity of Rhododendron in southern Yunnan, and no reproduction by biotechnological methods, so as to improve the reproduction coefficient, preserve and effectively utilize commercial value. , the effect of shortening the flowering period

Active Publication Date: 2015-01-21
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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  • Application Information

AI Technical Summary

Problems solved by technology

[0004] So far, there are no studies and reports on the propagation of rhododendrons in southern Yunnan related to biotechnology methods. In order to make rhododendrons in southern yunnan widely used in spice production and solve the problems of natural shortage and difficulty in introduction and domestication of rhododendrons in southern yunnan, a larger amount and faster To meet the needs of the market, it is necessary to study the tissue culture of this species and form a set of specialized tissue culture reproduction technology system

Method used

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  • Tissue culture propagation method for rhododendron hancockii
  • Tissue culture propagation method for rhododendron hancockii
  • Tissue culture propagation method for rhododendron hancockii

Examples

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Effect test

Embodiment 1

[0031] 1. Examples of media and matrix screening

[0032] Take the new tender stems of Rhododendron yunnanensis after flowering as explants, and disinfect them with conventional tissue culture explants. First, use 5ml detergent to fix the volume in 1L aqueous solution, soak and clean for 20min, and then rinse with sterile water After there is no foam, use 75% alcohol to sterilize for 20 seconds, rinse with sterile water for 3 times, add 2 drops of surfactant Tween-20 to 0.1% mercuric chloride solution for surface disinfection for 6 minutes, carry out twice, the first time Disinfect for 4 minutes, wash 5 times with sterile water after shaking continuously, then disinfect with 0.1% mercury liter for 2 minutes, and wash 5 times with sterile water. Cut off the browned tissue at both ends of the sterilized tender stem section, and cut it into stem sections of about 1 cm, with at least one axillary bud on each section. The stem segments were inoculated on WPM, Read, Anderson, and m...

Embodiment 2

[0047] The flowering twigs of Rhododendron yunnanensis were taken, brought back to the laboratory, and soaked in clean water. Take 5ml of detergent and dissolve it in 1L of tap water, soak the material in it for cleaning, and at the same time use a small brush to clean it. After 20 minutes, rinse it with sterile water until no foam is generated.

[0048] Bring the material to the ultra-clean bench in a clean container, sterilize it with 75% alcohol for 20 seconds, rinse it with sterile water for 3 times, configure 0.1% mercury liter solution (0.1g mercury liter to 1L) and add 2 drops on the surface The active agent Tween-20 (can be purchased at the biological reagent company) was used for surface disinfection for 6 minutes, which was carried out in two times. The first disinfection was performed for 4 minutes, and after continuous shaking, it was washed 5 times with sterile water, and then sterilized with 0.1% mercuric chloride for 2 minutes. Wash 5 times with sterile water an...

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Abstract

The invention discloses a tissue culture propagation method for rhododendron hancockii. The method is suitable for tissue culture quick propagation for ericaceae rhododendron hancockii and belongs to the technical field of plant biology. Bloomed fresh and tender stems are used as explants, and a series of steps of explant disinfection, cluster bud induction, subculture, seedling strengthening and ex vitro rooting are carried out; the problem that the quantity of plants is small due to a small rhododendron hancockii distribution region and difficulty in introduction and domestication and the problem that the requirement on commercial spice extraction cannot be met as the time for sowing seeds and flowering the seeds is long are effectively solved. According to the tissue culture propagation method, the propagation coefficient within one month is 10-20, the rooting rate is 80-90 percent, and the transplanting survival rate is 95-98 percent; the propagation coefficient of the rhododendron hancockii is greatly increased; a technical support is supplied for introduction and domestication, storage and commercial production of the species.

Description

technical field [0001] The invention relates to a method for plant tissue culture in plant biotechnology, in particular to a method for tissue culture and rapid propagation of rhododendron azalea in southern Yunnan. Background technique [0002] Rhododendron in southern Yunnan belongs to Rhododendron L., Subgen. Azaleastrum, Sect. Azaleastrum, Ericaceae family, and is an evergreen shrub. Rhododendron in southern Yunnan is white and has a strong fragrance. It is a good material for spice extraction and has high economic value. Due to the narrow distribution of rhododendron in southern Yunnan and limited materials, it is difficult to meet the raw material supply for spice production. [0003] At present, tissue culture rapid propagation has become an important means for the production of Chinese herbal medicines, flowers, and endangered species seedlings. It is reported that the adaptability of Rhododendron to the culture medium is closely related to the genotype of Rhododen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 马永鹏田晓玲罗桂芬黄承林
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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