Processing method of fish skin collagen polypeptide
A collagen polypeptide and processing method technology, applied in the field of polypeptide processing, can solve the problems of high-efficiency utilization and development of unfavorable polypeptide new products, low efficiency of filtration and impurity removal technology, and non-compliance with food safety, etc., to achieve guaranteed and guaranteed food safety The effect of high quality and safety
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Embodiment 1
[0021] Example 1: The thawed fish skin was used as raw material, cooked at 120° C. for 5 minutes, and then 6 times the weight of water was added for wet beating. Papain was selected for activity-oriented and controllable biological enzymatic hydrolysis. The enzymatic hydrolysis conditions were: 1% enzyme addition, 65°C temperature, and 5 hours to prepare collagen polypeptide enzymatic hydrolysis with high antioxidant activity. After the enzymatic hydrolysis is completed, inactivate the enzyme by incubating at 97°C for 5 minutes. After inactivating the enzyme, the crude collagen polypeptide enzymatic hydrolyzate was obtained by centrifuging in a continuous tubular centrifuge at 4000 rpm to remove impurities. Ultrafiltration membrane packs with molecular weight cut-offs of 10KDa, 5-10KDa, 1-5KDa and less than 1KDa are used for segmented cut-off, and collagen polypeptide solutions with 4 different molecular weight cut-offs can be obtained. And by controlling the ultrafiltration ...
Embodiment 2
[0022] Example 2: Using fresh fish skin as raw material, after cooking at a high temperature of 98° C. for 8 minutes, adding 3 times the weight of water for wet beating. The method of adding papain and compound protease at a ratio of 1:1 at the same time for activity-oriented and controllable biological enzymolysis can prepare peptides with high immune-modulating activity. The enzymolysis conditions were as follows: the total amount of enzyme added was 1.5%, the temperature was 60°C, and the time was 4 hours. After the enzymatic hydrolysis is completed, inactivate the enzyme by incubating at 95°C for 7 minutes. After inactivating the enzyme, the crude collagen polypeptide enzymatic solution was obtained by centrifuging in a continuous tubular centrifuge at 8,000 rpm to remove impurities. Ultrafiltration membrane packs with molecular weight cut-offs of 10KDa, 5-10KDa, 3-5KDa, 1-3KDa and less than 1KDa are used for segmental cut-off, and 5 kinds of collagen polypeptide solution...
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