Bio-slurry remediation method and special equipment for polybrominated diphenyl ether-contaminated soil
A technology of polybrominated diphenyl ethers and contaminated soil, which is applied in the field of bio-slurry remediation of polybrominated diphenyl ether-contaminated soils, can solve problems such as weakened thyroid function, achieve the effect of shortening the degradation time and improving the degradation effect
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Embodiment 1
[0033] Step 1): In this example, a bioslurry reactor made of stainless steel with a volume of 50L and equipped with stirring and aeration devices was used for the test; the seed tank for cultivating fungi and bacteria was a 15L fermenter with stirring and aeration devices ; The fungi used in this example are 1 strain of Phanerochaete chrysosporium, and the bacteria are 1 strain of Sphingomonas; the Sphingomonas that will be cultivated in the LB liquid medium is inoculated with 2% inoculum Carry out expanded cultivation in the bacterial seed tank; Simultaneously the Phanerochaete chrysosporium cultivated in the potato liquid medium is inoculated into the fungal seed tank with the amount of 2%; In the present embodiment, dress 30L polybrominated diphenyl ether contaminated soil, water and soil in the biological reaction The ratio is 3:1. Add inorganic salt nutrients to the bio-slurry reactor to adjust the content of inorganic salts in the reactor: Na 2 HPO 4 12H 2 o 7 .7.036...
Embodiment 2
[0039] Embodiment 2: polybrominated diphenyl ether polluted soil and water are 2:1 with water-to-soil volume ratio, adjust the inorganic salt content in the reactor: Na 2 HPO 4 12H 2 O6g / L, KH 2 PO 4 0.8g / L, (NH 4 ) 2 SO 4 0.3g / L,MgCl 2 ·6H 2 O 0.08g / L, Ca(NO 3 ) 2 4H 2 O40mg / L; trace element content FeSO 4 ·7H 2 O 2g / L, ZnSO 4 ·7H 2 O 0.02g / L,H 3 BO 3 0.2g / L, CoCl 3 ·6H 2 O0.2g / L, NiCl 2 ·6H 2 O0.02g / L,Na 2 MoO 4 2H 2 O 0.02g / L; Inoculate fungi and bacteria with polybrominated diphenyl ether degradation ability while reacting, the inoculum size is 5%; At a temperature of 25 ° C, the ventilation rate is 0.002m 3 / s, other with embodiment 1.
Embodiment 3
[0040] Embodiment 3: polybrominated diphenyl ether polluted soil and water are 4:1 with water-to-soil volume ratio, the inorganic salt content in the adjustment reactor: Na 2 HPO 4 12H 2 O 8g / L, KH 2 PO 4 1.2g / L, (NH 4 ) 2 SO 4 0.8g / L, MgCl 2 ·6H 2 O 0.12g / L,
[0041] Ca(NO 3 ) 2 4H 2 O460mg / L; trace element content FeSO 4 ·7H 2 O 3g / L, ZnSO 4 ·7H 2 O 0.04g / L, H 3 BO 3 0.4g / L, CoCl 3 ·6H 2 O0.3g / L, NiCl 2 ·6H 2 O 0.03g / L,Na 2 MoO 4 2H 2 O 0.04g / L, the inoculum size is 15%; the temperature is 30°C, and the ventilation volume is 0.008m 3 / s condition, others are the same as embodiment 1.
[0042] The microorganisms capable of degrading PBDEs described in the present invention can be obtained by screening PBDE-contaminated soils, or obtaining known high-efficiency degrading strains from bacterial strain preservation centers and research institutions in various countries. The fungi and bacteria inoculated in the bioreactor are first amplified and cultured...
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