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Compound biological fertilizer and preparation method thereof

A technology of compound biological fertilizer and plant lactobacillus, which is applied in the field of compound biological fertilizer and its preparation, can solve problems such as backward production technology, excessive bacteria, and loss of the role of biological fertilizer

Inactive Publication Date: 2015-01-28
邵素英
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, with the development of bio-fertilizers, in a few places, there are no production licenses, backward production technology, production with unidentified strains, low effective bacteria content, and excessive bacteria in the production of bio-fertilizers in a few places.
There are also some manufacturers who add inappropriate amount of chemical fertilizers or other additives to biological fertilizers, so that the nitrogen, phosphorus, and potassium content of chemical fertilizers in fertilizers are too high, resulting in the death of effective bacteria and losing the role of biological fertilizers.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0063] (1) Preparation of bacterial suspension

[0064] Inoculate the Li-2013 single colony grown after streaking on the plate into the seed medium, culture at 40°C for 12 hours at 100 r / min, take 1 mL of the culture medium, centrifuge, wash twice with normal saline, and resuspend with 9 mL of physiological in salt water.

[0065] (2) Ultraviolet-Lithium Chloride-Diethyl Sulfate Compound Mutagenesis

[0066] The bacterial suspension was placed on a sterile plate, stirred and irradiated for 100s under a UV lamp with a distance of 30cm and a power of 15w. The irradiated bacteria solution was diluted and spread on the lithium chloride plate after gradient dilution, and the bacteria solution without ultraviolet irradiation was diluted and spread on the plate as a control. Wrap the uniformly coated plate above with black cloth or newspaper, incubate at 40°C for 48 hours, screen out the plate with the largest ratio of hydrolysis circle to colony diameter on the plate that grows co...

Embodiment 1

[0102] A compound biological fertilizer is composed of Aspergillus niger culture, Bacillus megaterium phosphorus solubilizing, Bacillus subtilis and Lactobacillus plantarum inoculum. Bacillus 5 parts, Bacillus subtilis bacterial agent 20 parts, Lactobacillus plantarum CGMCC NO.940515, polyglutamic acid 4.

[0103] The polyglutamic acid is γ-polyglutamic acid.

Embodiment 2

[0105] A compound biological fertilizer is composed of Aspergillus niger culture, Bacillus megaterium phosphorus solubilizing, Bacillus subtilis and Lactobacillus plantarum inoculum. Bacillus 8 parts, Bacillus subtilis bacterial agent 25 parts, Lactobacillus plantarum CGMCC NO.940510, polyglutamic acid 3.

[0106] The polyglutamic acid is γ-polyglutamic acid.

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Abstract

The invention discloses a compound biological fertilizer and a preparation method thereof and relates to a novel biological fertilizer and production thereof. The compound biological fertilizer comprises the following components in parts by weight: 10-25 parts of an aspergillus niger culture, 3-8 parts of phosphate-solubilizing bacillus megaterium, 5-25 parts of a bacillus subtilis bacterial agent, 10-25 parts of lactobacillus plantarum CGMCC NO. 9405 and 3-5 parts of polyglutamate. The polyglutamate is gamma-polyglutamate. By using the product disclosed by the invention, the yield of grains can be improved by 20-40%, the yield of vegetables can be improved by 20-40%, and the quality and flavor of the vegetables and grains can be improved.

Description

Technical field: [0001] The invention relates to fertilizers in agricultural technology, and relates to a novel biological fertilizer and its production. Background technique: [0002] The evolution of biological fertilizers from the initial rhizobia agent to bacterial fertilizers to today's biological fertilizers has explained the gradual development of biological fertilizers in my country. At present, more than 70 countries in the world have produced, applied and promoted biological fertilizers. At present, there are about 500 enterprises in my country with an annual output of about millions of tons of biological fertilizers used in production. Although this is not comparable to the output and amount of chemical fertilizers used in the same period, it has indeed begun to play a role in agricultural production and has achieved certain economic and social effects. [0003] Biological fertilizer refers to a special type of fertilizer that contains a large number of living mi...

Claims

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Application Information

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IPC IPC(8): C05F11/08
CPCC05C11/00C05F11/08
Inventor 邵素英李建树
Owner 邵素英