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A kind of extraction, separation and purification method of γ-aminobutyric acid

A technology for the separation and purification of aminobutyric acid, applied in the field of separation and purification, can solve the problems of impractical production and use, high cost, low yield of γ-aminobutyric acid, etc., and achieve easy promotion and use, easy preparation process, and purification method Effects in simple steps

Active Publication Date: 2016-03-02
四川肽美生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Technical problem to be solved: In the conventional methods for extracting, separating and purifying γ-aminobutyric acid from rice, most of them are laboratory operation methods, limited by their high cost, they cannot be actually used in industrial production, and the preparation The yield of gamma-aminobutyric acid is lower, which increases the use cost of gamma-aminobutyric acid, so there is a need for an extraction, separation and purification method that is easy to use industrially and has a high yield of prepared gamma-aminobutyric acid

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] (1) Crush the rice, add 40wt% ethanol aqueous solution for extraction after smashing, the extraction temperature is 50°C, the ratio of solid to liquid is 1:7, the extraction time is 3h, and the extract is centrifuged after extraction , the centrifugal speed is 4000rpm, and the supernatant is taken;

[0018] (2) Concentrate the supernatant to twice the weight of the original rice, adjust the pH of the supernatant to 4.5 after concentration, pass through the activated 732 cation exchange resin, the sample loading flow rate is 1BV / h, and the resin column diameter to height ratio is 8:1;

[0019] (3) After the sample loading, the 732 cation exchange resin was eluted with ammonia water with a concentration of 0.03mol / L at a flow rate of 0.5BV / h. After elution, the eluate was collected and concentrated. After concentration, no solvent remains, and then purify the concentrate with ethanol. The purification temperature is at room temperature. The weight ratio of ethanol to the...

Embodiment 2

[0022] (1) Crush the rice, add 30wt% ethanol aqueous solution for extraction after smashing, the extraction temperature is 45°C, the ratio of solid to liquid is 1:6, the extraction time is 3h, and the extract is centrifuged after extraction , the centrifugal speed is 4000rpm, and the supernatant is taken;

[0023] (2) Concentrate the supernatant to twice the weight of the original rice, adjust the pH of the supernatant to 5 after concentration, pass through the activated 732 cation exchange resin, the sample loading flow rate is 2BV / h, and the diameter-to-height ratio of the resin column is 8:1;

[0024] (3) After loading the sample, use ammonia water with a concentration of 0.02mol / L to elute the 732 cation exchange resin at a flow rate of 1BV / h, collect the eluate after elution, concentrate the eluate, and concentrate Finally, no solvent remains, and then purify the concentrate with ethanol, the purification temperature is room temperature, and the weight ratio of ethanol t...

Embodiment 3

[0027] (1) Crush the rice, add 50wt% ethanol aqueous solution for extraction after smashing, the extraction temperature is 55°C, the ratio of solid to liquid is 1:8, the extraction time is 3h, and the extract is centrifuged after extraction , the centrifugal speed is 4000rpm, and the supernatant is taken;

[0028] (2) Concentrate the supernatant to twice the weight of the original rice, adjust the pH of the supernatant to 4 after concentration, pass through the activated 732 cation exchange resin, the sample loading flow rate is 1.5BV / h, and the diameter of the resin column is high The ratio is 8:1;

[0029] (3) After loading the sample, the 732 cation exchange resin was eluted with ammonia water with a concentration of 0.04mol / L at a flow rate of 1.5BV / h. After elution, the eluate was collected and concentrated. After concentration, no solvent remains, and then purify the concentrate with ethanol. The purification temperature is at room temperature. The weight ratio of ethan...

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PUM

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Abstract

The invention belongs to the fields of extraction, separation and purification, and discloses a method for extracting, separating and purifying gamma-aminobutyric acid. The method comprises the following steps: 1, triturating rice, adding an ethanol-water solution to the triturated rice to extract, centrifuging the obtained extraction liquid, and taking the obtained supernatant; 2, concentrating the supernatant, adjusting the pH value of the concentrated supernatant, and adding the pH adjusted concentrated supernatant to activated 732 cation exchange resin; and 3, eluting the 732 cation exchange resin by ammonia water, collecting the obtained eluate, concentrating the eluate, and purifying the obtained eluate concentrate by ethanol three times to obtain gamma-aminobutyric acid.

Description

technical field [0001] The invention belongs to the field of separation and purification, and relates to a method for extracting, separating and purifying gamma-aminobutyric acid, in particular to a method for extracting, separating and purifying gamma-aminobutyric acid in rice. Background technique [0002] Gamma-aminobutyric acid (GABA) is a natural amino acid that is not composed of proteins. GABA is an important inhibitory neurotransmitter in the central nervous system of mammals and has important physiological functions: (1) Improve brain function and prolong memory. Studies have shown that GABA can increase the activity of glucose phospholipase, thereby promoting energy metabolism in the animal brain, activating cerebral blood flow, increasing oxygen supply, and ultimately restoring brain cell function and improving neurological function. (2) Improve visual function. According to reports, an electrode with a capillary was inserted into the visual cortex of the brain ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C227/40C07C229/08
Inventor 张小英
Owner 四川肽美生物科技有限公司