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Method for determining content of saccharide components in ixeri sonhifolia injection

A measurement method and injection technology, applied in measuring devices, material separation, analysis of materials, etc., can solve the problems of lack of quality detection methods, etc., and achieve easy-to-operate sample pre-treatment steps, simple sample pre-treatment steps, and ensure precision Effect

Active Publication Date: 2015-01-28
TONGHUA HUAXIA PHARMA
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] According to literature reports, Kudiezi contains sugar components, but there is no quality detection method for this substance in the existing literature and reports

Method used

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  • Method for determining content of saccharide components in ixeri sonhifolia injection
  • Method for determining content of saccharide components in ixeri sonhifolia injection
  • Method for determining content of saccharide components in ixeri sonhifolia injection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0033] Determination method of carbohydrate content in Kudiezi injection:

[0034] 1. Instruments and reagents

[0035] 1.1 Instrument

[0036] GC-MS-QP2010SE (SHIMADZU company, Japan).

[0037] HH-S8 digital display constant temperature water bath (Jinfang Shenglan Instrument Manufacturing Co., Ltd.).

[0038] SHZ-95B circulating water multi-purpose vacuum pump (Gongyi City Yuhua Instrument Co., Ltd.).

[0039] Freeze dryer (FD5-2.5, Japan).

[0040] BT125D analytical balance (Sartorius, Germany).

[0041] High-speed centrifuge (TG16-WS Changsha Xiangyi Centrifuge Instrument Co., Ltd.).

[0042] 1.2 Materials

[0043] D-glucose anhydrous reference substance (National Institute for the Control of Pharmaceutical and Biological Products 110833-200904).

[0044] D-allose reference substance (Tianjin Yifang Technology Co., Ltd. 101205003).

[0045] D-galactose reference substance (China Institute for the Control of Pharmaceutical Biology 100226-200404).

[0046] D-mannos...

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Abstract

The invention relates to an identification method for a traditional Chinese injection, and particularly relates to a method for determining the content of saccharide components in an ixeri sonhifolia injection by using GC-MS. The method comprises the following steps of (a) preparation of a sample, comprising a pretreatment method of the sample; (b) preparation of a standard substance, comprising a derivatization method of aldose components and a derivatization method of ketose components; (c) chromatographic conditions: a GC-MS analyzer employs a GC-MS-QP2010SE and capillary column is shown in the description; and (d) determination: taking the standard substance and the sample precisely, injecting into a gas chromatograph, determining by gas chromatography, and making a fingerprint spectrum of the saccharide components in the ixeri sonhifolia injection. With the method, the difficultly detectable saccharide components in an ixeri sonhifolia can be detected accurately by adopting the derivatization method.

Description

technical field [0001] The invention relates to a method for identifying traditional Chinese medicine injections, in particular to a method for determining sugar components in Kudiezi injection by using GC-MS. Background technique [0002] Kudiezi is a perennial herb of the family Asteraceae. In recent years, as traditional Chinese medicine has been paid more and more attention, the research on the chemical composition, pharmacological activity and clinical research application of Kudiezi has made great progress. Kudiezi injection is a light yellow-brown to yellow-brown clear liquid, clinically used for coronary heart disease, angina pectoris, cerebral thrombosis, cerebral infarction, etc. [0003] The chemical composition of Kudiezi is relatively complex. According to literature reports, its main components include terpenoids, flavonoids, coumarins, and sterols. Among them, the main active ingredients are affected by factors such as the place of origin, climate and ecolog...

Claims

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Application Information

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IPC IPC(8): G01N30/88
Inventor 王晖张艳君李遇伯刘东辉陈峰
Owner TONGHUA HUAXIA PHARMA
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