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Medical appliance containing recombinant hCREG protein and preparation method of medical appliance

A medical device and protein technology, which is applied in the field of medical devices containing recombinant hCREG protein and its preparation, can solve the problem of low expression level, achieve the effects of inhibiting cell proliferation, avoiding allergic and inflammatory reactions, and promoting re-endothelialization

Inactive Publication Date: 2015-02-04
LEPU MEDICAL TECH (BEIJING) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Further studies have found that CREG is a small-molecular-weight secreted glycoprotein, which is widely expressed in differentiated mature tissue cells, but the expression level is very low in undifferentiated tissues such as embryonic stem cells and teratoma cells

Method used

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  • Medical appliance containing recombinant hCREG protein and preparation method of medical appliance
  • Medical appliance containing recombinant hCREG protein and preparation method of medical appliance
  • Medical appliance containing recombinant hCREG protein and preparation method of medical appliance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Example 1 Preparation of recombinant hCREG glycoprotein and its coronary stent

[0052] 1. Preparation of Microporous Recombinant hCREG Glycoprotein Elution Scaffold

[0053] 1.1 Preparation of recombinant hCREG glycoprotein

[0054] 1.1.1 Construction of recombinant gene vector Since 1999, the mRNA differential display technology was used to successfully clone the human CREG gene from the human internal thoracic artery VSMCs cultured in vitro for the first time, and the hCREG open reading frame with a stop codon mutation was amplified by RT-PCR technology Frame, the hCREG RT-PCR primers of the stop codon mutation are as follows: the upstream primer is 5'-aa ggatcc atggccgggctatcccgc-3' (SEQ ID NO.1), and the downstream primer is: 5'-gc gaattc Gcactgaactgtgacatttaatattcttctgg-3' (SEQ ID NO .2), where the capital letter represents the stop codon of the C / G mutation.

[0055] The recombinant human CREG protein expression vector pcDNA3.1-his / myc-hCREG with his tag protei...

Embodiment 2

[0066] Embodiment 2 Preparation of drug-protein combined scaffold

[0067] The surface of the coronary stent in this embodiment is uniformly distributed with recombinant hCREG glycoprotein and rapamycin. Rapamycin is distributed on the outer layer of the stent, and the recombinant hCREG glycoprotein of the present application is distributed in the lumen of the stent.

[0068] Stent with micro-pores: the stent is made of 316L medical stainless steel substrate, and the surface is uniformly equipped with micro-pores of about 500 nm.

[0069] Preparation of three-sided rapamycin drug stent: prepare rapamycin drug with a concentration of 1% in acetone solution. The inner surface of the stent is protected, and rapamycin is sprayed on the outer surface of the stent.

[0070] Preparation of recombinant hCREG protein-eluting scaffolds: the recombinant hCREG glycoprotein is immobilized to the inner lumen of the scaffold by using the principle of physical adsorption of micron holes. T...

Embodiment 3

[0072] Example 3 Preparation of double-drug protein combined scaffold

[0073] The surface of the coronary stent in this embodiment is uniformly distributed with recombinant hCREG glycoprotein, rapamycin and paclitaxel. Rapamycin and paclitaxel were distributed on the outer surface of the stent, and recombinant hCREG glycoprotein was distributed in the lumen of the stent.

[0074] Stent with micro-pores: the stent is made of 316L medical stainless steel substrate, and the surface is uniformly equipped with micro-pores of about 500 nm.

[0075] Prepare a three-sided rapamycin drug stent: protect the inner surface of the stent, spray rapamycin and paclitaxel on the outer surface of the stent, the drug concentration is 1%, the drug solvent adopts tetrahydrofuran, and the space between rapamycin and paclitaxel is The mass ratio is 1:1.

[0076] Preparation of recombinant hCREG protein-eluting stent (drug on the outer surface, protein on the inner surface): the recombinant hCREG ...

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Abstract

The invention provides a medical appliance containing recombinant hCREG glycoprotein. The recombinant hCREG glycoprotein is uniformly applied to the surface of the medical appliance, and can be combined with multiple drugs for using. After the medical appliance is implanted in a human body, by means of constant release of the hCREG glycoprotein carried by the appliance, cell proliferation of vascular smooth muscle can be inhibited, re-endothelialization of vascular walls can be promoted, and injured vascular wall can be quickly cured, so that occurrence of thrombus in the cavity of the medical appliance can be prevented, and the time of taking anti-thrombus and blood platelet medicines after a patient is operated can be reduced.

Description

technical field [0001] The invention relates to bioengineering and the preparation of bioengineering medical equipment, in particular to a medical equipment containing recombinant hCREG protein and a preparation method thereof. It belongs to the field of bioengineering and interventional medical devices implanted in the body. technical background [0002] Cardiovascular disease is a serious threat to human health. According to the WHO report, the number of deaths due to cardiovascular disease is as high as 30% of the total number of deaths in the world every year. The main trend in the treatment of severe cardiovascular diseases during cardiovascular interventional implantation operations, but interventional valves, occluders, stents and other medical devices are likely to cause the formation of thrombus and vegetation, and vascular stenosis (restenosis, RS), which affects the success of the operation rate, significantly reducing the benefit of surgery. [0003] Studies ha...

Claims

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Application Information

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IPC IPC(8): A61L31/16A61L31/10C12N15/85C07K14/47
CPCA61L31/10A61L31/16C07K14/47C12N15/85
Inventor 韩雅玲张娜邓婕闫承慧孙鸣宇陈永强崔凯蒲忠杰
Owner LEPU MEDICAL TECH (BEIJING) CO LTD
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