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Method for detecting dipterex and acephate by virtue of enzyme labelled bionic immunoassay

A technology of acephate and acephate labeling, which is applied in the field of fast and sensitive detection of trichlorfon and acephate by enzyme-labeled biomimetic immunoassay with multiple residues of organophosphorus pesticides, and can solve the problem of difficult preparation of specific antibodies , Pesticide detection types are single, expensive instruments and other problems, to achieve high sensitivity, overcome the effect of long preparation cycle and cost reduction

Inactive Publication Date: 2015-02-04
SHANDONG AGRICULTURAL UNIVERSITY
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Problems solved by technology

[0004] The purpose of the present invention is to overcome the shortcomings of traditional organophosphorus pesticide detection methods such as long detection time, expensive instruments, difficult preparation of specific antibodies, and single type of pesticide detection, and provide an enzyme-labeled bionic immunoassay for the detection of trichlorfon and acetylformazan Aminophos method

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  • Method for detecting dipterex and acephate by virtue of enzyme labelled bionic immunoassay

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Embodiment Construction

[0022] Below in conjunction with embodiment, the present invention is further described; Following embodiment is illustrative, not limiting, can not limit protection scope of the present invention with following embodiment.

[0023] The present invention uses the general organophosphorus pesticide hapten as a template molecule to synthesize a molecularly imprinted polymer film as a bionic antibody and applies it to enzyme-labeled immunoassay technology, thereby establishing a biomimetic immunoassay method for organophosphorus general-purpose hapten enzyme-labeled bionic immunoassay, which is used for vegetable Detection of trichlorfon and acephate in agricultural products. Its specific embodiment is:

[0024] 1. Synthesis of common haptens of organophosphorus pesticides: Add 0.01mol 4-aminobutyric acid to 10mL 2.5mol / L sodium hydroxide solution in an ice bath at 4°C, stir magnetically for 30min, then add 1.215mLO, O'- Dimethyl thiophosphoryl chloride was stirred at room tempe...

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Abstract

The invention relates to a method for detecting dipterex and acephate by virtue of enzyme labelled bionic immunoassay. The method comprises the steps of the synthesis of half antigens, the preparation of enzyme-labelled antigens, the preparation of a multiple-recognition site hydrophilic molecular imprinted polymer membrane, the establishment of a standard curve and the like. According to the method for detecting the dipterex and the acephate by virtue of the enzyme labelled bionic immunoassay, the prepared hydrophilic imprinted polymer membrane is taken as bionic antibody to take the place of a biological antibody, and a high-sensitivity detection method having a wide detection range for the dipterex or the acephate is established by use of the immunosorbent assay principle. The defects of long preparation cycle, easiness in inactivation, high cost and the like of the traditional biological antibody are overcome and the analysis time can be greatly shortened. The method for detecting the dipterex and the acephate by virtue of the enzyme labelled bionic immunoassay is low in cost, simple in experimental operation, high in sensitivity, and suitable for quick detection on the dipterex or the acephate in various agricultural products.

Description

technical field [0001] The invention relates to an enzyme-labeled bionic immunoassay method for detecting trichlorfon and acephate, which belongs to the technical field of food safety detection, in particular to a rapid, rapid and effective method for detecting trichlorfon and acephate and other organophosphorus pesticides with multiple residues. Sensitive detection method. Background technique [0002] Organophosphorus pesticides are a class of organic compound pesticides that act on crops to kill and prevent pests and diseases. Toxicology experiments have shown that it will cause neurological diseases after entering the human and animal bodies through the digestive tract, respiratory tract and other channels, and has neurotoxicity. Some organophosphorus pesticides will form new substances after transformation. For example, trichlorfon will become dichlorvos after transformation, which will increase the toxicity and cause greater harm to the environment and animals. [00...

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Application Information

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IPC IPC(8): G01N33/53G01N33/535
CPCG01N33/53G01N33/535
Inventor 徐志祥时辰高绘菊
Owner SHANDONG AGRICULTURAL UNIVERSITY
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