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Molecular mark of rice brown planthopper inhibition major gene qBph29(t) and application of molecular mark

A technology of anti-BPH and main gene, applied in the field of plant molecular genetics, can solve problems such as difficult to effectively introduce and aggregate anti-insect genes, complex identification of insect resistance, etc., and achieve convenient identification, simple detection methods, and clear selection targets Effect

Active Publication Date: 2015-02-11
GUANGXI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Due to the complexity of insect resistance identification, it is often difficult to effectively introduce and aggregate different insect resistance genes using conventional breeding methods

Method used

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  • Molecular mark of rice brown planthopper inhibition major gene qBph29(t) and application of molecular mark
  • Molecular mark of rice brown planthopper inhibition major gene qBph29(t) and application of molecular mark
  • Molecular mark of rice brown planthopper inhibition major gene qBph29(t) and application of molecular mark

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1: Acquisition of Molecular Markers

[0025] (1) 186S / 08BPH327F 2 Population construction and phenotyping

[0026] (1) In the previous research, researcher Li Rongbai of Guangxi University screened a large number of wild rice materials with high resistance to brown planthopper from a large number of common wild rice materials in Guangxi. Through hybridization and backcrossing with cultivated rice varieties, combined with the identification of brown planthopper resistance, The resistance traits were transferred to cultivated rice materials, and after multi-generation self-crossing and insect resistance identification, rice lines with high resistance to brown planthopper and stable agronomic traits were screened. Among them, the new resistance source 08BPH327 is the progeny material of the hybridization and backcrossing of the common wild rice that is resistant to brown planthoppers and Xixiangnuo. The insect resistance identification experiment shows that 08BPH3...

Embodiment 2

[0052] Example 2: Verification of Molecular Markers

[0053] 1. Materials and methods

[0054] 1.1 Materials

[0055] Negative varieties: 30 copies, susceptible lines (species) 186S, 9311, Nipponbare, Taichung Native No. 1, Baimao and Bai R54, all of which are conventional rice materials preserved in our laboratory, offspring of 186S×08BPH327 or 9311×08BPH327 hybrid combinations 24 susceptible families in the

[0056] Positive varieties: 29 insect-resistant lines in the progeny of high insect-resistant lines 08BPH327, 186S×08BPH327 or 9311×08BPH327 hybrids.

[0057] YM70 is a molecular marker designed and developed based on the corresponding sequences of 08BPH327 and 9311 genomes.

[0058] The upstream primer of the primer pair for amplifying the molecular marker is the sequence shown in Seq ID NO.1, and the downstream primer of the primer pair is the sequence shown in Seq ID NO.2;

[0059] Seq ID NO.1: 5'-CTCTCCCCTCCAATCCTTTT-3'

[0060] Seq ID NO.2: 5'-AGCACCCTGGAAAGCAG...

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Abstract

The invention provides a close linkage molecular mark of a rice brown planthopper inhibition major gene qBph29(t) and application of the molecular mark. According to the genetype of a single F2 strain obtained from hybridization of rice insect resistant lines 08BPH327 (male parent) and 9311 (female parent), and meanwhile together with genetic linkage analysis on the brown planthopper inhibition level of a F2:3 family, the molecular mark which is closely linked with qBph29(t) is found in a 120K area between molecular marks RM16720 and YM54 of the insect resistant line 08BPH327 positioned resistance major gene qBph29(t). By adopting the molecular mark, whether the insect resistant line 08BPH327 and a derivative variety of the insect resistant line 08BPH327 have the major resistance genetic loci or not can be effectively detected, the selection efficiency of rice brown planthopper inhibition plants is greatly improved, and a rice brown planthopper inhibition variety containing qBph29(t) is obtained.

Description

technical field [0001] The invention belongs to the field of plant molecular genetics, and relates to a molecular marker of a rice main gene qBph29(t) resistant to brown planthopper and the application of the molecular marker in breeding rice varieties resistant to brown planthopper. Background technique [0002] Rice is one of the most important food crops in my country. In recent decades, it has been damaged by a large number of diseases and insect pests, which seriously threatens its production. Nilaparvata lugens is a monophagous pest. Its adults and nymphs use needles to suck the phloem juice of plants, causing yellow leaves or withering of rice plants, and finally leading to yield reduction or failure. According to the China Agricultural Yearbook, rice planthoppers (mainly brown planthoppers) occurred nationwide in 1966, 1969, 1973, 1977, 1983 and 2003, and in 1987, 1991, 2005, 2006 and 2007. The area of ​​harm reaches more than 50% of the total rice planting area, whi...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12N15/10C12Q1/68
Inventor 邱永福李容柏刘芳杨萌覃宝祥
Owner GUANGXI UNIV
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