Method for obtaining human adipose-derived stem cells and construction method for multilevel allogeneic adipose-derived stem cell bank

A technology of human adipose stem cells and adipose stem cells, which is applied in the field of obtaining adipose stem cells, can solve the problems of stem cell aging and large damage to cell activity, and achieve the effects of small cell damage, reducing the damage process, and simplifying the process

Inactive Publication Date: 2015-02-18
天晴干细胞股份有限公司
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  • Summary
  • Abstract
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  • Application Information

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Problems solved by technology

[0008] The present invention aims to solve the problem that the existing method for obtaining human adipose stem cells and the large damage to cell activity when collecting fat, and the stem cells in the existing adipose ste

Method used

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  • Method for obtaining human adipose-derived stem cells and construction method for multilevel allogeneic adipose-derived stem cell bank
  • Method for obtaining human adipose-derived stem cells and construction method for multilevel allogeneic adipose-derived stem cell bank
  • Method for obtaining human adipose-derived stem cells and construction method for multilevel allogeneic adipose-derived stem cell bank

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Experimental program
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specific Embodiment approach 1

[0046] Specific embodiment one: the method for obtaining human adipose stem cells in this embodiment is carried out according to the following steps:

[0047] 1. Screening of donors:

[0048] Donors are required to be in good health. HCV, HBsAg, HBcAb, CMV, HIV, Syphilis, HLTV and EBV virus tests in the blood of the donor are negative, and the tests for anaerobic bacteria, aerobic bacteria, broad-spectrum mycoplasma and fungi in the blood are all negative. Patients with past medical history, family genetic disease history, drug abuse history, no inflammation, no wound healing difficulties and poor compliance were excluded. If the donor was a woman, it was required to be in a non-pregnant and non-menstrual state, and the donor had no need for anesthetics. allergic reaction;

[0049] 2. Fat collection:

[0050]Fat collection is performed on the donors screened in step 1. The fat collection adopts hydrodynamic liposuction technology with a negative pressure of 200-350mmHg. Afte...

specific Embodiment approach 2

[0055] Embodiment 2: This embodiment is different from Embodiment 1 in that: the age of the donor described in Step 1 is 18-40 years old. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0056] Embodiment 3: This embodiment differs from Embodiment 1 or Embodiment 2 in that: the negative pressure strength of the hydrodynamic liposuction technique in step 2 is 210-300 mmHg. Others are the same as in the first or second embodiment.

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Abstract

The invention discloses a method for obtaining human adipose-derived stem cells and a construction method for a multilevel allogeneic adipose-derived stem cell bank, relates to an adipose-derived stem cell obtaining method and an adipose-derived stem cell bank construction method, and aims to solve the problems of great damage to cell activity caused by a fat collection method and a conventional human adipose-derived stem cell obtaining method and high aging rate of stem cells used by multiple persons after subculture of many times in a conventional adipose-derived stem cell bank. The method for obtaining the stem cells comprises the following steps of 1, screening donors; 2, collecting fats; 3, transporting the fats; 4, separating the human adipose-derived stem cells. The construction method for the stem cell bank comprises the following steps of 1, screening the donors; 2, collecting the fats; 3, transporting the fats; 4, separating the human adipose-derived stem cells; 5, culturing, subculturing and amplifying the human adipose-derived stem cells; 6, constructing a main cell bank; 7, establishing working cell banks. According to the methods, the cell activity is less damaged, and multiple levels of stem cell banks for multiple persons are established for the establishment of the adipose-derived stem cell bank.

Description

technical field [0001] The invention relates to a method for obtaining adipose stem cells and a method for constructing an adipose stem cell bank. Background technique [0002] Adipose-Derived Stem Cells (ADSCs or Adipose-Derived Mesenchymal Stromal cells, ADMSCs) are a type of stem cells with multidirectional differentiation potential obtained from adipose tissue, which can differentiate into osteoblasts under specific induction conditions. cells, chondrocytes, and adipocytes. In 2001, Zuk first obtained such stem cells with cell therapy potential by separating human adipose tissue, thus pioneering the research and application of adipose stem cells. After more than 10 years of scientific research and practice, it has been found that adipose stem cells are a group of cells with regeneration and differentiation capabilities similar to bone marrow mesenchymal stem cells. They do not express CD34, CD45 and other hematopoietic cell surface antigens, so they will not differentia...

Claims

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Application Information

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IPC IPC(8): C12N5/0775
Inventor 张怡王灵娟芦慧颖王浩宇梁丽潮付寅生赵新新
Owner 天晴干细胞股份有限公司
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