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Black fungus liquid fermentation quick-producing high-yield melanin culture medium

A technology for liquid fermentation and culture medium, applied in the directions of fermentation, microorganism-based methods, microorganisms, etc., can solve the problems of low melanin production capacity, and achieve the effects of high melanin production, less mycelial growth, and improved melanin production.

Inactive Publication Date: 2015-02-18
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the microbial resources for producing natural melanin are very rich, and many strains have been discovered and reported to produce melanin, the industrialization of microbial melanin production has not been realized so far. Low

Method used

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  • Black fungus liquid fermentation quick-producing high-yield melanin culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1: Effects of Different Nutrient Conditions on the Growth Characteristics of Mycelia and the Regularity of Melanin Formation

[0016] 1 Materials and methods

[0017] 1.1 Experimental materials

[0018] 1.1.1 Strains

[0019] Black Fungus( A. auricula ) (Sun Shujing et al. Screening of black fungus strains producing natural pigment and research on their physical and chemical properties. Journal of Jiangxi Agricultural University, 2010, 32(1): 160).

[0020] 1.1.2 Medium

[0021] (1) Enriched PDA solid medium: glucose 20 g / L, yeast powder 5 g / L, peptone 3 g / L, KH 2 PO 4 3 g / L, MgSO 4 1.5 g / L, VB 1 0.1 g / L, agar 20 g / L, pH natural.

[0022] (2) Liquid complete medium: lactose 10 g / L, yeast extract 10 g / L, tyrosine 1 g / L, CaCl 2 0.1 g / L, NaCl 5 g / L.

[0023] (3) Carbon-free medium: yeast extract 10 g / L, tyrosine 1 g / L, CaCl 2 0.1 g / L, NaCl 5 g / L.

[0024] (4) Nitrogen-free medium: lactose 10 g / L, tyrosine 1 g / L, CaCl 2 0.1 g / L, NaCl 5 g / L. ...

Embodiment 2

[0050] Example 2: Optimization of Fermentation Conditions for Black Fungus to Produce Melanin

[0051] 1 Materials and methods

[0052] 1.1 Experimental materials

[0053] 1.1.1 Strains

[0054] Black Fungus( A. auricula ) to preserve strains for this laboratory.

[0055] 1.1.2 Medium

[0056] (1) PDA liquid medium: potato 200 g / L, glucose 20 g / L, pH natural.

[0057] (2) PDA solid medium: potato 200 g / L, glucose 20 g / L, agar 20 g / L, pH natural.

[0058] (3) Enriched PDA solid medium: glucose 20 g / L, yeast powder 5 g / L, peptone 3 g / L, KH 2 PO 4 3 g / L, MgSO 4 1.5 g / L, VB 1 0.1 g / L, agar 20 g / L, pH natural.

[0059] (4) Tyrosine fermentation medium: glucose 1 g / L, peptone 5 g / L, CaCl 2 0.1 g / L, NaCl 5 g / L, tyrosine 1 g / L, pH 7.0.

[0060] 1.2 Experimental method

[0061] 1.2.1 Activation of test strains

[0062] The preserved strains were inoculated into enriched PDA slant, and cultured at 28°C for 5 days. The newly grown slant was transferred to enriche...

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Abstract

The invention relates to a black fungus liquid fermentation quick-producing high-yield melanin culture medium. The formula of the culture medium comprises the following raw materials: 17.27 g / L of a yeast extract, 1.92 g / L of tyrosine, 3.84 g / L of lactose, 1 g / L of NaCl, 2 g / L of MgSO4, 0.5 mg / L of biotin, and 1 g / L of KH2PO4, wherein the pH value is 6. According to the invention, the melanin is produced by black fungus liquid fermentation; with optimization of the fermentation conditions, the melanin production by fermentation can be ahead of time, and the yield of melanin can be increased. Under the optimized conditions, the yield of melanin can be up to 2.97 g / L, which is 2.14 times more than that of the existing culture medium; melanin can be produced on the third day during the culture process. The formula of the culture medium provided by the invention aims to shorten the melanin production time; the mycelial growth amount is less, and the melanin yield is high; the culture medium is more favorable to separate out and extract melanin, so as to reduce the follow-up working cost.

Description

technical field [0001] The invention specifically relates to a black fungus liquid fermentation fast-production and high-yield melanin medium. The medium formula has the characteristics of early melanin production and high melanin output. Background technique [0002] Melanin is a kind of polymer compound formed by oxidative polymerization of phenols or indoles, which can be produced in animals, plants and microorganisms. Melanin is not a necessary condition for the growth and development of organisms, but it plays a protective function for organisms against environmental stress, such as ultraviolet radiation and extreme temperature, so it can improve the ability of organisms to survive and compete. In addition, melanin can also be used in cosmetics manufacturing, free radical scavenging, and biopesticide photoprotection, etc., and its application prospects are very broad, so the study of natural melanin is of great significance. [0003] In addition to black, natural melan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/02C12R1/645
Inventor 孙淑静张晓娟胡开辉张燎原杨云龙
Owner FUJIAN AGRI & FORESTRY UNIV
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