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Bacillus with blocking effect on cadmium and its application

A bacillus and effect technology, applied in the field of bacteria and microorganisms, can solve the problems of occupying the planting area of ​​food crops and low efficiency, and achieve the effects of obvious barrier effect, efficient prevention of cadmium entering the plant body, significant adsorption and immobilization effect

Active Publication Date: 2017-07-28
SICHUAN LANYUE SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Plant extraction is enrichment through hyperaccumulation plants, and the purpose of removing heavy metals is achieved by collecting plants, but the efficiency is low and it takes up the planting area of ​​food crops

Method used

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  • Bacillus with blocking effect on cadmium and its application
  • Bacillus with blocking effect on cadmium and its application
  • Bacillus with blocking effect on cadmium and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Bacillus ( Bacillus sp. ) The preparation method of LY152, the specific operation is as follows:

[0030] (1) Sample collection: In October 2012, 0-20cm soil samples of cadmium-contaminated soil in Shifang City, Sichuan Province were collected.

[0031] (2) Enrichment culture: Weigh 10 g of soil sample into sterilized liquid medium, culture in a 28°C incubator with shaking for 48 hours, then absorb 10 ml of culture solution for subculture, and pass 5 times. The composition of the liquid medium is: sucrose 10 g L -1 , K 2 HPO 4 2 g L -1 , (NH 4 ) SO 4 1 g L -1 , MgSO 4 ·7H 2 O 0.5 g L -1 , CaCO 3 0.5 g L -1 , yeast extract 0.5 g·L -1 , NaCl0.1 g L -1 , CdCl 2 2.5H 2 O 0.2 g L -1 , pH 7.0.

[0032] (3) Isolation of cadmium-resistant bacterial strains: draw 10ml of enriched culture solution into a triangular flask filled with 90ml of sterile normal saline, oscillate on a shaker at 28°C for 30min, then draw 1ml into 9ml of sterile normal saline and gradu...

Embodiment 2

[0040] The application of Bacillus to reduce the entry of heavy metal cadmium into rice, the specific operation is as follows:

[0041] 1. Materials

[0042] Strains: Bacillus ( Bacillus sp. ) LY152, the registration number of the General Microorganism Center (CGMCC) of China Microbiological Culture Collection Management Committee is CGMCC NO.9541

[0043] Planting medium: heavy metal cadmium polluted soil, cadmium pollution concentration 1 mg·L -1 and 40 mg·L -1

[0044] Planting container: Cultivation pot with a volume of 50 liters

[0045] Plant seeds: rice seeds, the varieties are: Fuyou 838, Nuoxiang 61

[0046] 2. Operation method and steps

[0047] (1) Seedling raising: rice seeds are raised in the greenhouse, and the leaf age is about 3 leaves and 1 heart can be transplanted;

[0048] (2) Bacterial liquid preparation: the effective bacillus ( Bacillus sp. ) LY152 for 1 x 10 9 Inoculate in 2 liters of liquid medium per milliliter, shake and culture at 150 rpm a...

Embodiment 3

[0055] The application of Bacillus in reducing the entry of heavy metal cadmium into vegetables, the specific operation is as follows:

[0056] 1. Materials

[0057] Strains: Bacillus ( Bacillus sp. ) LY152, the registration number of the General Microorganism Center (CGMCC) of China Microbiological Culture Collection Management Committee is CGMCC NO.9541

[0058] Planting medium: heavy metal cadmium polluted soil, cadmium pollution concentration 40 mg·L -1

[0059] Planting container: Cultivation pot with a volume of 2 liters

[0060] Vegetable Seedlings: Cabbage Seedlings

[0061] 2. Operation method and steps

[0062] (1) Bacteria solution preparation: same as Example 1;

[0063] (2) Planting medium preparation: pollute the soil with 2 kg of cadmium per pot;

[0064] (3) Seedling transplanting: transplant pakchoi seedlings into the medium prepared in step (2), using the method of applying 4mL of bacterial solution to the root of each seedling, and setting no bacteria...

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Abstract

The invention is a bacillus having a blocking effect on cadmium, which solves the problem that cadmium enters the food chain. Its Latin name is Bacillus sp, depository unit: General Microorganism Center of China Microbiological Culture Collection Management Committee, deposit date: August 25, 2014, deposit number: CGMCC NO.9541. The active effect of the bacterial strain on cadmium can prevent the heavy metal cadmium from entering the plant body, thereby reducing the cadmium pollution from entering the food chain, and is particularly suitable for the actual planting conditions in my country where the polluted soil is planted and repaired.

Description

[0001] Technical field: [0002] The invention relates to a barrier effect on soil heavy metal cadmium pollution, which can reduce the bacteria and microorganisms that cadmium enters the food chain. [0003] technical background: [0004] Cadmium (Cd) is a heavy metal element with strong biological toxicity. It has strong chemical activity, high mobility, and long-lasting toxicity in the environment. It is easy to endanger human health through the enrichment of the food chain, and can induce liver, kidney, and bone lesions. There are also carcinogenic effects. The area of ​​cultivated land polluted by cadmium in my country has reached 1.3×10 4 hm 2 , about 5.0×10 per year 7 kg of rice cadmium content exceeded the standard. In 2003, the Rice and Product Quality Supervision, Inspection and Testing Center of the Ministry of Agriculture conducted a general survey on the quality and safety of rice in various places in my country, which showed that according to the Cd content o...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10C12R1/07
CPCB09C1/10C12N1/205C12R2001/07
Inventor 侯勇王强锋张亮夏中梅朱彭玲黄秀辉胡甦王贇曾芸黄双飞李红兵王兰英曾显斌
Owner SICHUAN LANYUE SCI & TECH
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