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Peanut vitamin C synthesis related gene Ah PMM and application thereof

A technology of vitamins and genes, applied in the fields of application, genetic engineering, plant genetic improvement, etc., can solve the problems such as the gaps in functional research reports, and achieve the effect of improving salt tolerance and increasing the vitamin C content of leaves

Active Publication Date: 2015-02-25
QINGDAO AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, it has been cloned in Arabidopsis, tobacco, soybean, rice, tomato, wheat, etc. PMM gene, but there is no report of cloning in peanut, and there are still gaps in the report of the functional study and transgene regulation of this gene in peanut

Method used

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  • Peanut vitamin C synthesis related gene Ah PMM and application thereof
  • Peanut vitamin C synthesis related gene Ah PMM and application thereof
  • Peanut vitamin C synthesis related gene Ah PMM and application thereof

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Embodiment 1

[0031] Example 1: Genes related to peanut vitamin C synthesis AhPMM and its application in improving vitamin C content and salt tolerance

[0032] 1. Experimental materials

[0033] 1. Genes, strains and peanut varieties

[0034] The present invention clones peanut PMM Gene( AhPMM ), Escherichia coli DH5α, and Agrobacterium tumefaciens strain EHA105 are preserved by the Genetics Research Laboratory of Qingdao Agricultural University, and engineering strains commercialized by biological companies can also be used. The transgenic recipient material is the peanut variety "Junan Xiaohongzhong" (provided by the Laboratory of Genetics, Qingdao Agricultural University).

[0035] The cloned peanut AhPMM The gene sequence list is shown in SEQ ID No:1, and the amino acid sequence of the encoded protein is shown in SEQ ID No:2.

[0036] clone the peanut AhPMM The primer names and sequences of the genes are as follows:

[0037] P1 (5'-AAATGGCTGCCCGGAAACCTGG-3') (SEQ ID No: 4);

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Abstract

The invention provides a peanut vitamin C synthesis related gene Ah PMM and an application thereof. A transgenic plant of which the total vitamin C and reduction state vitamin C (AsA) content is significantly improved can be obtained after the gene is subjected to over-expression in peanuts. Proved by experiments, the vitamin C content of peanut leaves can be significantly improved by performing over-expression on the Ah PMM gene provided by the invention without causing significant influences to normal growth of the peanuts. Protein and an encoding gene thereof provided by the invention have important theoretical and practical significance in research of synthesis mechanisms of plant vitamin C, improvement for increasing the plant vitamin C content and stress resistance, and are wide in application prospect.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to genes related to peanut vitamin C synthesis AhPMM and its application. Background technique [0002] Vitamin C, namely ascorbic acid, plays a regulatory role in the oxidation-reduction metabolic reaction of organisms, and human beings lack it can cause scurvy. In addition, it also has other functions. For example, lack of vitamin C, cholesterol cannot be hydroxylated into bile acid to be excreted; its oxidase will affect the metabolism of certain amino acids (generally aromatic); it also has detoxification effects, etc. Generally, vitamin C can be synthesized by itself in animals and plants, but ascorbic acid cannot be synthesized in the human body. This is due to the lack of gurolactone oxidase in the human body. The vitamin C synthesized by the plant itself can not only be used by humans, but also play an important role in the physiological functions of the plant itself. Gener...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/61C12N9/90A01H5/00
Inventor 隋炯明郑春花王亚赵春梅孔祥远王晶珊乔利仙
Owner QINGDAO AGRI UNIV
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