Bacillus amyloliquefacien FS6 and application thereof

A technology of amylolytic spores and FS6, which is applied in the field of agricultural microorganisms, can solve the problems of untimely control and loss, and achieve the effects of enhancing defense capabilities, strong colonization capabilities, and reducing residues

Active Publication Date: 2015-03-04
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since 2002, the incidence of ginseng gray mold has been increasing year by year in the ginseng

Method used

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  • Bacillus amyloliquefacien FS6 and application thereof
  • Bacillus amyloliquefacien FS6 and application thereof
  • Bacillus amyloliquefacien FS6 and application thereof

Examples

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preparation example Construction

[0033] The preparation method of bacillus amyloliquefaciens FS6 bacterial agent of the present invention comprises the following steps:

[0034] (1) Strain activation: Inoculate Bacillus amyloliquefaciens FS6 on a solid medium and culture it at 28°C for 1-2 days;

[0035] (2) Fermented seed culture: inoculate the above-mentioned activated bacterial strains in liquid medium, and shake and culture in a shaker at 180r / min for 24 hours at 28°C to obtain a seed liquid;

[0036] (3) Bacteria fermentation: the seed solution was added to the KMB medium, and cultured in a shaker at 180 r / min for 36 hours at 28° C. to prepare the Bacillus amyloliquefaciens FS6 bacterial agent.

[0037] The formula of the solid medium for the activation culture of the above-mentioned step (1) strain is: beef extract 5.0g, peptone 10.0g, NaCl 5.0g, distilled water 1000mL, agar powder 15g, pH7.0.

[0038] The formula of the liquid medium used for the cultivation of the fermented seeds in the above step (2...

Embodiment 1

[0045] Example 1 Isolation, screening and identification of Bacillus amyloliquefaciens FS6

[0046] 1.1 Isolation of bacteria

[0047]The soil samples collected from the main production areas of ginseng were separated by dilution separation method. Take 3g of soil samples and add them into 30mL of sterile water, incubate with shaking at 240r / min, 28°C for 30min, then take them out for use. Under sterile conditions, the soil suspension was serially diluted to 10 with sterile water -3 , 10- 4 、10 -5 three concentration gradients. 100 μL was taken successively and spread on the surface of NA medium, and each treatment was repeated three times, and placed in a constant temperature incubator at 28°C for 48 hours. Observe regularly during the cultivation period, select the bacterial colonies with dominant colonies for purification, and save them for future use.

[0048] Beef extract peptone medium (NA): 10g peptone, 3g beef extract, 5g NaCl, 15g agar powder, 1000mL distilled w...

Embodiment 2

[0058] The preparation of embodiment 2 bacillus amyloliquefaciens FS6 fermented liquid

[0059] (1) Strain activation: Inoculate Bacillus amyloliquefaciens FS6 on a solid medium and culture it at 28°C for 1-2 days;

[0060] (2) Fermented seed culture: inoculate the above-mentioned activated bacterial strains in liquid medium, and shake and culture in a shaker at 180r / min for 24 hours at 28°C to obtain a seed liquid;

[0061] (3) Bacteria fermentation:

[0062] By 6% inoculum size, above-mentioned seed liquid is inoculated in inoculum fermentation culture liquid, under the condition of 28 ℃, 180r / min shakes and cultivates 36h in the shaker, makes the inoculum of bacillus amyloliquefaciens FS6 (number of viable bacteria) About 3.0×10 9 cfu / ml).

[0063] The formula of solid medium for strain activation culture is: beef extract 5.0g, peptone 10.0g, NaCl 5.0g, distilled water 1000mL, agar powder 15g, pH7.0.

[0064] The formula of the liquid culture medium for strain fermented...

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Abstract

The invention relates to bacillus amyloliquefacien FS6 and application thereof. The preservation number of the bacillus amyloliquefacien FS6 is CGMCC No. 9538. The invention also provides a preparation method of the bacillus amyloliquefacien FS6 inoculant. The bacillus amyloliquefacien FS6 disclosed by the invention and an inoculant thereof have higher bacteriostatic activity on pathogenic bacteria in ginseng roots and leaves, and can induce the activity of ginseng peroxidase, polyphenol oxidase, phenylalnine ammonialyase and other enzymes relating to defense to rise so as to improve the resistance of ginseng to pathogenic bacteria. The bacillus amyloliquefacien FS6 and the inoculant thereof can be stably colonized in ginseng plants and soil, so that a protective film is formed on the surface of an object to be applied to prevent pathogenic bacteria from invasion, and the occurrence of ginseng roots and leaf diseases is effectively reduced. The bacillus amyloliquefacien FS6 has no phytotoxicity on ginseng and no environment pollution, and has a good biopesticide development potential and an application prospect.

Description

technical field [0001] The invention relates to the technical field of agricultural microorganisms, in particular to a bacillus amyloliquefaciens FS6 and an application thereof. Background technique [0002] Ginseng (Panax ginseng C.A. Meyer), as a precious Chinese herbal medicine, has the largest cultivation area and the highest yield in my country. my country has become the main ginseng producer and exporter in the world. The annual ginseng output accounts for more than 78% of the world's total output, and the export volume accounts for more than 60% of the international market, but the economic benefits produced are very low. China's ginseng output is about three times that of South Korea, but its output value is only 1 / 4 of that of South Korea. This is mainly due to the excessive pesticide residues in ginseng and the unreasonable use of pesticides. [0003] Ginseng has a long growth period, generally 4 to 6 years. At the same time, ginseng has high requirements for prod...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/07
CPCA01N63/00A01N63/10C12N1/205C12R2001/07
Inventor 高洁王春伟刘丽萍王燕白庆荣杨丽娜陈长卿欧师琪王雪
Owner JILIN AGRICULTURAL UNIV
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