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Blood alanine aminotransferase detecting reagent with high stability

A technology of alanine amino group and detection reagent, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve problems such as attenuation, poor repeatability, and inaccurate results, and achieve improved stability, slowed attenuation, and operation Simple and convenient effect

Active Publication Date: 2015-03-11
BIOBASE BIODUSTRY (SHANDONG) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The former is easy to operate and does not require special instruments and reagents, but the results are not accurate enough, the repeatability is poor, and the effect is not ideal; the latter is a well-recognized method with accurate results, but it has certain requirements for instruments and reagents
At present, the double-reagent enzyme-coupled continuous detection method is mostly used in the market to detect serum ALT, and the operation steps are cumbersome, while the single-reagent enzyme-coupled continuous detection method is simple and convenient to operate, but the enzyme and NADH in the reagent are unstable. It is easy to cause attenuation, which brings great difficulties to the detection of serum ALT by single reagent method

Method used

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  • Blood alanine aminotransferase detecting reagent with high stability
  • Blood alanine aminotransferase detecting reagent with high stability
  • Blood alanine aminotransferase detecting reagent with high stability

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] A highly stable alanine aminotransferase detection reagent, the reagent consists of:

[0048] Tris buffer (pH=7.8) 40mmol / L

[0049] L-alanine 400mmo1 / L

[0050] Potassium chloride 1g / L

[0051] EDTA.Na 2 5mmol / L

[0052] Trehalose 1g / L

[0053]Bovine Serum Albumin (BSA) 1g / L

[0054] Dithiothreitol 0.1g / L

[0055] EMERGEN A-60 0.1g / L

[0056] Proclin-300 0.5ml / L

[0057] α-ketoglutarate 10mmo1 / L

[0058] Lactate dehydrogenase (LDH) 1000U / L

[0059] NADH 0.15mmo1 / L.

[0060] The preparation method of the above-mentioned reagent is as follows: Tris, L-alanine, potassium chloride, trehalose, BSA, EDTA.Na 2 , dithiothreitol, EMERGEN A-60 and Proclin-300 were dissolved in 1L of deionized water in sequence according to the formula, and the pH of the solution was adjusted to 7.8 with sodium hydroxide, and then lactate dehydrogenase (LDH) and NADH were added in sequence Dissolved in the above solution to complete the preparation.

Embodiment 2

[0062] A highly stable alanine aminotransferase detection reagent, the reagent consists of:

[0063] Tris buffer (pH=7.8) 60mmol / L

[0064] L-alanine 600mmo1 / L

[0065] Potassium chloride 10g / L

[0066] EDTA.Na 2 10mmol / L

[0067] Trehalose 10g / L

[0068] Bovine Serum Albumin (BSA) 10g / L

[0069] Dithiothreitol 10g / L

[0070] EMERGEN A-60 10g / L

[0071] Proclin-300 1ml / L

[0072] α-ketoglutaric acid 20mmo1 / L

[0073] Lactate dehydrogenase (LDH) 14000U / L

[0074] NADH 0.25mmo1 / L.

[0075] The reagent preparation method of this embodiment is the same as that of Example 1.

Embodiment 3

[0077] A highly stable alanine aminotransferase detection reagent, the reagent consists of:

[0078] Tris buffer (pH=7.8) 50mmol / L

[0079] L-alanine 500mmo1 / L

[0080] Potassium chloride 8g / L

[0081] EDTA.Na 2 10mmol / L

[0082] Trehalose 6g / L

[0083] Bovine Serum Albumin (BSA) 6g / L

[0084] Dithiothreitol 6g / L

[0085] EMERGEN A-60 6g / L

[0086] Proclin-300 1ml / L

[0087] α-ketoglutarate 15mmo1 / L

[0088] Lactate dehydrogenase (LDH) 1200U / L

[0089] NADH 0.18mmo1 / L.

[0090] The preparation method of the reagent of this embodiment is the same as that of Example 1.

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Abstract

The invention discloses a blood alanine aminotransferase detecting reagent with high stability, and belongs to the technical field of clinical in-vitro detection. According to the blood alanine aminotransferase detecting reagent with high stability, disclosed by the invention, the blood serum alanine aminotransferase (ALT) is detected by using the single reagent method on the basis of an enzyme-coupled continuous detection method, so that the problem that the single reagent in the original formula is unstable is solved, and the reagent is simple and convenient to operate, and facilitates clinical application and popularization.

Description

technical field [0001] The invention relates to a highly stable blood alanine aminotransferase detection reagent, which belongs to the technical field of clinical in vitro detection. Background technique [0002] Alanine aminotransferase (ALT) is an enzyme involved in human protein metabolism (equivalent to a catalyst in industrial production), which plays a role in accelerating the transformation of protein amino acids in the body. It widely exists in various tissues, organs, Among the muscles and bones, the cytoplasm of liver cells is the most. The reference value of the normal content of ALT in serum is: 9-51U / L for men and 8-41U / L for women (the test methods of each hospital are different, and the reference values ​​are not exactly the same ). When various tissues and organs in the human body are active or diseased, the ALT in them will be released into the blood, increasing the serum ALT content. For example, when the liver is inflamed, the transaminases will be relea...

Claims

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Application Information

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IPC IPC(8): C12Q1/52C12Q1/32
Inventor 杨君莉罗维晓甘宜梧谭柏清王绮朱玉娥肖慧
Owner BIOBASE BIODUSTRY (SHANDONG) CO LTD
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