PBLD gene expression primer for primary hepatoma treatment scheme selection and/or prognosis assessment, and application thereof

A technology for hepatocellular carcinoma and treatment plan, which is applied in the determination/examination of microorganisms, recombinant DNA technology, biochemical equipment and methods, etc., can solve the problems of poor prognosis of liver cancer, lack of treatment methods, etc. Treatment effect, effect of avoiding statistical analysis

Inactive Publication Date: 2015-03-11
广州市第一人民医院
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Problems solved by technology

Therefore, the prognosis of liver cancer is poor, and there is still a lack of effective treatment methods.
[0004] At present, there is no efficient and specific molecular marker to judge the prognosis of liver cancer

Method used

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  • PBLD gene expression primer for primary hepatoma treatment scheme selection and/or prognosis assessment, and application thereof
  • PBLD gene expression primer for primary hepatoma treatment scheme selection and/or prognosis assessment, and application thereof
  • PBLD gene expression primer for primary hepatoma treatment scheme selection and/or prognosis assessment, and application thereof

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Embodiment Construction

[0021] A total of 108 pairs of liver cancer resected by hepatobiliary surgery and corresponding paracancerous liver tissues (more than 2 cm from the tumor margin) were collected, and the clinical manifestations, imaging and postoperative pathology were consistent with the diagnosis of primary hepatocellular carcinoma. The clinical characteristics of 108 patients with liver cancer are shown in Table 1. Among them, 24 patients were lost to follow-up, so a total of 84 patients were included in the survival analysis. The follow-up time ranged from 12 to 62 months, with a median follow-up time of 44 months. TNM staging was based on the seventh edition of the American Joint Committee on Cancer (AJCC) cancer staging manual.

[0022] Table 1108 clinical characteristics statistics of patients with liver cancer:

[0023]

[0024] According to the detection results of real-time fluorescence quantitative PCR, the patients whose expression level of PBLD mRNA in liver cancer tissues was ...

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Abstract

The invention discloses a PBLD gene expression primer for primary hepatoma treatment scheme selection and / or prognosis assessment, and an application thereof. The primer comprises an upstream primer 5'-GGGTCTGCACACGCTGTTC-3' and a downstream primer 5'-TAATGTCAACCCTTCCGTCT-3'. Prognosis can be accurately assessed through PBLD detection on a primary hepatoma patient or a biopsy tissue by using the specific primer and a kit made via using the primer according to the PBLD gene expression level in order to avoid tedious statistical analysis, and accurate curative effect assessment can be provided for a clinic doctor and the patient according to detection results in order to determine the suitable treatment scheme of the patient, guide the doctor to select an effective clinic treatment scheme, improve the treatment effect of the patient and realize individual treatment.

Description

technical field [0001] The present invention relates to a primer, in particular to a primer for detecting PBLD gene expression used in the selection of treatment scheme and / or prognosis assessment of primary hepatocellular carcinoma, and also relates to the application of the primer. Background technique [0002] The full-length human PBLD gene is 2575bp, located on human chromosome 10, and widely expressed in brain, heart, lung, liver, pancreas and other tissues. At present, in the genomics study of the gene expression profile of liver cancer, it is found that the expression of PBLD is lower or absent than that of the normal liver tissue adjacent to the cancer. However, the significance of the abnormal expression of PBLD in liver cancer tissue in clinical prognosis has not been reported. Primary liver cancer (Primary hepatocellular carcinoma, HCC) is one of the most common malignant tumors in humans, with the characteristics of high malignancy, easy metastasis and recurren...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6886C12Q2600/118C12Q2600/158
Inventor 李爱民燕群聂玉强
Owner 广州市第一人民医院
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