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Direct bilirubin detection reagent

A technology for detecting reagents and bilirubin, which is applied in the direction of material analysis by observing the influence on chemical indicators, and analysis by making materials undergo chemical reactions, which can solve the problems of high price, short storage time and accurate impact of enzymatic methods. Sexuality and other problems, to achieve the effect of eliminating the interference of interfering substances and indirect bilirubin, high clinical application value, and strong anti-interference ability

Active Publication Date: 2015-03-11
BIOBASE BIODUSTRY (SHANDONG) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the methods for measuring serum bilirubin mainly include diazo method, enzymatic method and chemical oxidation method. The sulfonic acid is temporarily prepared, and the storage time is short, and the specificity of the reaction between the diazo reagent and the indirect bilirubin and direct bilirubin is not good. When the direct bilirubin is determined under certain conditions, there is a small amount of indirect Participate in the reaction, if the reaction time is prolonged, more people will participate, resulting in inaccurate measured values, the price of the enzymatic method is more expensive, and the activity of bilirubin oxidase is also prone to decline, which affects the accuracy of the measurement
[0004] Chemical oxidation methods include vanadate oxidation and nitrite oxidation, which are susceptible to indirect bilirubin, such as free bilirubin, delta-bilirubin, etc. In clinical practice, false positives are prone to occur, affecting the accuracy of test results, and thus affecting the application of reagents in the market

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] A direct bilirubin detection reagent comprises reagent R1 and reagent R2 with a volume ratio of 4:1;

[0048] Reagent R1 includes the following components:

[0049] Tartrate buffer (pH=2.0) 0.05mol

[0050] Hydrazine Hydrochloride 10mol

[0051] Surfactant 10mL

[0052] Proclin 300 1mL

[0053] Add deionized water to 1L;

[0054] Reagent R2 includes the following components:

[0055] Phosphate buffer (pH=7.5) 100mmol

[0056] Sodium metavanadate 1mmol

[0057] Proclin 300 1mL

[0058] Make up to 1 L with deionized water.

[0059] The surfactant is composed of sodium dodecylbenzenesulfonate: fatty alcohol ester-35: triethanolamine: betaine = 1:1:1:1 volume ratio.

[0060]The method of using the direct bilirubin detection reagent in this example is as follows: select Toshiba 40 automatic analyzer with double reagent function, prepare reagent R1 and reagent R2, place reagent R1 and reagent...

Embodiment 2

[0065] A direct bilirubin detection reagent comprises reagent R1 and reagent R2 with a volume ratio of 4:1;

[0066] Reagent R1 includes the following components:

[0067] Tartrate buffer (pH=3.0) 0.07mol

[0068] Hydrazine hydrochloride 8mol

[0069] Surfactant 2mL

[0070] Proclin300 6mL

[0071] Add deionized water to 1L;

[0072] Reagent R2 includes the following components:

[0073] Phosphate buffer (pH=6.5) 75mmol

[0074] Sodium metavanadate 8mmol

[0075] Proclin300 5mL

[0076] Make up to 1 L with deionized water.

[0077] The surfactant is composed of sodium dodecylbenzenesulfonate: fatty alcohol ester-35: triethanolamine: betaine = 1:1:1:1 volume ratio.

[0078] A method of using the direct bilirubin detection reagent is the same as in Example 1.

Embodiment 3

[0080] A direct bilirubin detection reagent comprises reagent R1 and reagent R2 with a volume ratio of 4:1;

[0081] Reagent R1 includes the following components:

[0082] Tartrate buffer (pH=4.0) 0.1mol

[0083] Hydrazine hydrochloride 5mol

[0084] Surfactant 1mL

[0085] Proclin300 10mL

[0086] Add deionized water to 1L;

[0087] Reagent R2 includes the following components:

[0088] Phosphate buffer (pH=6.0) 50mmol

[0089] Sodium metavanadate 10mmol

[0090] Proclin300 10mL

[0091] Make up to 1 L with deionized water.

[0092] The surfactant is composed of sodium dodecylbenzenesulfonate: fatty alcohol ester-35: triethanolamine: betaine = 1:1:1:1 volume ratio.

[0093] A method of using the direct bilirubin detection reagent is the same as in Example 1.

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Abstract

The invention discloses a direct bilirubin detection reagent. The detection reagent disclosed by the invention comprises a reagent R1 and a reagent R2 of which the volume ratio is 4:1, wherein the reagent R1 comprises tartaric acid buffer solution, hydrazine dihydrochloride, a surfactant and Proclin300; the reagent R2 comprises phosphate buffer solution, sodium metavanadate and Proclin300. The direct bilirubin detection reagent disclosed by the invention has high anti-interference capacity, high accuracy, higher linearity and high stability, can eliminate interference of interfering substances and indirect bilirubin, is suitable for a full-automatic biochemical analyzer and has high clinical application value.

Description

technical field [0001] The invention belongs to the technical field of clinical in vitro detection, in particular to a direct bilirubin detection reagent. Background technique [0002] Direct bilirubin is also called conjugated bilirubin. Unconjugated bilirubin is converted in liver cells and combined with glucuronic acid to form conjugated bilirubin. Conjugated bilirubin has a direct reaction with van den Berg qualitative test, so this bilirubin is called direct bilirubin . Determination of direct bilirubin is mainly used to identify the type of jaundice. Although there are many reasons for high direct bilirubin, the most common one is high direct bilirubin caused by liver disease. Therefore, when direct bilirubin is found to be high, the first thing to consider is whether you have liver disease, conduct a comprehensive analysis based on the patient's medical history, symptoms, etc., select appropriate inspection items, determine the cause of direct bilirubin high, and t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
Inventor 靖百谦赵峰玉甘宜梧谭柏清王绮谢清华
Owner BIOBASE BIODUSTRY (SHANDONG) CO LTD
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