Rapid detection method of varicella-zoster virus titer
A technology of varicella virus and detection method, which is applied in the biological field, can solve problems such as inability to survive for a long time and instability of varicella zoster virus, and achieve the effects of saving detection time, shortening the detection cycle, and improving detection efficiency
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Embodiment 1
[0034] A rapid detection method for varicella virus titer, the detection method utilizes the initial stage of varicella virus to infect monolayer cells, replicates in infected cells and expresses a large amount of varicella virus envelope glycoproteins related to virus pathogenicity, through the method of immune response , determine the infection focus, observe and count under a microscope and calculate the titer of the varicella virus;
[0035] The detection method comprises the following steps:
[0036] 1) Virus inoculation
[0037] Take human normal embryonic lung fibroblasts (MRC-5 cells), cover a single layer of a 96-well plate, make gradient dilutions of the varicella virus samples to be tested, take 2 to 4 wells as a group, and add the same concentration of the varicella virus to each group. Virus dilution 25ul, make parallel wells and negative control wells at the same time, gently shake the plate to distribute the varicella virus evenly, 25~45℃, absorb for 30~90 minu...
Embodiment 2
[0054] This embodiment is a preferred solution on the basis of embodiment 1. The quality of the raw materials used is the same as that of embodiment 1, and the same part as embodiment 1 will not be repeated. Please refer to embodiment 1.
[0055] The rapid detection method of varicella virus titer comprises the following steps:
[0056] 1) Virus inoculation
[0057] Human normal embryonic lung fibroblasts (MRC-5 cells) were collected and covered with a single layer of 96-well plate, 3×10 per well 4 cells, incubate at 25-45°C for 1 hour, suck out the medium with a pipette, and be careful not to hang the cells;
[0058] Do gradient dilution of the varicella virus sample to be tested, take 2 wells as a group, add 25ul of the varicella virus diluent of the same concentration to each group, make parallel wells and negative control wells at the same time, shake the plate gently to make the varicella virus evenly Distribution, 25 ~ 45 ° C, adsorption for 60 minutes, gently shake on...
Embodiment 3
[0076] This embodiment is a preferred solution on the basis of embodiment 1. The quality of the raw materials used is the same as that of embodiment 1, and the same part as embodiment 1 will not be repeated. Please refer to embodiment 1.
[0077] The rapid detection method of varicella virus titer comprises the following steps:
[0078] 1) Virus inoculation
[0079] Human normal embryonic lung fibroblasts (MRC-5 cells) were collected and covered with a single layer of 96-well plate, 3.5×10 per well 4 cells, incubate at 25-45°C for 1 hour, suck out the medium with a pipette, and be careful not to hang the cells;
[0080] Make a gradient dilution of the varicella virus sample to be tested, take 3 wells as a group, add 25 μl of the varicella virus dilution solution of the same concentration to each group, and make 2 parallel wells at the same time, and add 25 μl negative control wells to the other 2 wells, gently Shake the plate to distribute the varicella virus evenly, adsorb ...
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