Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for comprehensively utilizing cartilage to realize clean extraction of chondroitin sulfate

A chondroitin sulfate and cleaning technology, applied in the chemical industry, can solve the problems of large ethanol consumption, long cycle, black and smelly organic wastewater, etc., and achieve remarkable economic and social benefits

Inactive Publication Date: 2015-03-18
QINGDAO LANNONGGU AGRI PROD RES & DEV
View PDF1 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are three main methods for the production of chondroitin sulfate in China: salt hydrolysis, alkali hydrolysis and enzymatic hydrolysis. The method used more in China is to extract chondroitin sulfate by combining dilute alkali or concentrated alkali with enzymatic hydrolysis. The alkaline enzymatic hydrolysis production process has cumbersome steps and a long cycle, generally takes 3-5 days, and the product quality and yield are unstable. In the process of high-temperature cooking, alkaline extraction, enzymatic hydrolysis and decolorization, chondroitin sulfate is severely damaged. Low product yield, darkened solution color, large ethanol consumption, high production cost
[0004] At present, a large amount of animal protein, bone oil, bone residue and its degradation products produced in the production process of chondroitin sulfate are discharged without utilization. The untreated organic wastewater is black and smelly, and eutrophic, which brings serious pollution to the water environment.
At present, only a small number of relatively large-scale chondroitin sulfate production enterprises treat sewage, and generally adopt simple physical and chemical treatment as the main terminal treatment method, that is, a large amount of protein pollutants are all concentrated in the tail treatment, and the one-time investment is relatively large, and the operation cost High, the protein has not been utilized, and it is difficult to meet the discharge standard

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Select 1000kg of chicken leg bones, crush them into pieces below 1cm, put them in a vacuum tank, add 800kg of distilled water, control the vacuum in the tank to 0.02MPa, and heat at 70°C for 2 hours; put the pretreatment solution into the enzymatic hydrolysis tank to adjust the pH To 7-8, add 0.8% of cartilage mass with trypsin, pepsin and papain at a ratio of 1:1:1. During the enzymolysis process, the pH of the solution should be constantly adjusted to keep it at 7.5-8.0, and the temperature should be kept at 35°C. Decompose for 4 hours; after enzymatic hydrolysis, use a disc-type three-phase centrifuge at a speed of 6500r / min for 15 minutes to separate the upper layer to obtain 50kg of bone oil; after crushing the bone residue, obtain 760kg of bone powder. Concentrate the centrifuged filtrate through a three-effect concentrator. During the concentration process, control the temperature of the first effect at 90°C, the second effect at 85°C, and the third effect at 75°C...

Embodiment 2

[0029] Select 1000kg of chicken leg bones, crush them into pieces below 2cm, put them into a vacuum tank, add 900kg of distilled water, control the vacuum in the tank to 0.04MPa, and heat at 80°C for 3 hours; put the pretreatment solution into the enzymatic hydrolysis tank to adjust the pH To 7-8, add trypsin, pepsin and papain with a ratio of 1:2:1 of 0.8% of the cartilage mass. During the enzymolysis process, the pH of the solution should be constantly adjusted to keep it at 7.5-8.0, and the temperature should be kept at 40°C. Decompose for 6 hours; after enzymatic hydrolysis, use a disc-type three-phase centrifuge at a speed of 6500r / min, centrifuge for 20 minutes, and separate the upper layer to obtain 50kg of bone oil; after crushing the bone residue, obtain 765kg of bone powder. Concentrate the centrifuged filtrate through a three-effect concentrator. During the concentration process, control the temperature of the first effect at 95°C, the second effect at 90°C, and the ...

Embodiment 3

[0031]Select 1000kg of chicken leg bones, crush them into pieces below 1cm, put them into a vacuum tank, add 900kg of distilled water, control the vacuum in the tank to 0.03MPa, and heat at 75°C for 3 hours; put the pretreatment solution into the enzymatic hydrolysis tank to adjust the pH To 7-8, add trypsin, pepsin and papain with a ratio of 1:1:2 of 1.0% of the cartilage mass. During the enzymolysis process, the pH of the solution should be constantly adjusted to keep it at 7.5-8.0, and the temperature should be kept at 40°C. Decompose for 6 hours; after enzymatic hydrolysis, use a disc-type three-phase centrifuge at a speed of 6500r / min, centrifuge for 20min, and separate the upper layer to obtain 50kg of bone oil; after crushing the bone residue, obtain 757kg of bone powder. Concentrate the centrifuged filtrate through a three-effect concentrator. During the concentration process, control the temperature of the first effect at 93°C, the second effect at 87°C, and the third ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for comprehensively utilizing cartilage to realize clean extraction of chondroitin sulfate. The method comprises the steps of (1) cartilage hydrolysis pretreatment, (2) enzymatic hydrolysis, (3) centrifugation to obtain bone oil and bone meal, (4) concentration, (5) adsorption elution, (6) chondroitin sulfate preparation and (7) protein peptide preparation. A composite enzyme hydrolysis technology is adopted to realize synchronous hydrolysis of chondroitin sulfate and protein peptides. Chondroitin sulfate release and hydrolysis of the protein peptides in cartilage are simultaneously carried out, and a large amount of proteins and other pollutants generated in traditional chondroitin sulfate production are recycled to realize synchronous production of chondroitin sulfate, the protein peptides, bone oil and bone meal, comprehensive utilization of cartilage, energy saving, emission reduction, and substantial economic and social benefits.

Description

technical field [0001] The invention belongs to the technical field of chemical industry, and in particular relates to a method for comprehensively utilizing cartilage to clean and extract chondroitin sulfate. Background technique [0002] Chondroitin sulfate is an acidic mucopolysaccharide extracted from cartilage such as mammalian trachea. White powder, odorless, tasteless, easy to absorb moisture, soluble in water, insoluble in organic solvents such as ethanol and acetone, it swells or becomes viscous when it meets water, and is relatively unstable to heat. It needs to be stored away from light and sealed. Chondroitin is a mucopolysaccharide composed of D-glucuronic acid and N-acetyl-D-galactosamine. Chondroitin sulfate is the sulfate ester of chondroitin, a major component of connective tissue. It has the functions of clarifying lipids, improving the detoxification function of the body, diuresis and analgesia. my country's chondroitin sulfate is exported to the Unite...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/08C12P21/06
Inventor 李妮
Owner QINGDAO LANNONGGU AGRI PROD RES & DEV