Butterfly orchid tissue culture seedling out vitro rooting method
A technology of rooting and tissue culture seedlings outside the test tube, applied in the field of plant tissue culture, can solve the problems of large indoor space occupation, long seedling cultivation cycle, high production cost, etc., and achieve the effects of cost saving, high rooting rate and high survival rate
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Embodiment 1
[0018] 1) Experiment specific operation:
[0019] In 2013, the experiment was carried out according to the following dates: strong seedling cultivation was carried out in late May, transplanting was carried out on June 6, and survival rate statistics were carried out on August 19. The seedlings of the three varieties Dajiao, Wenjingangang, and Liulin Black Rose were cultured for 10-15 days. When the tissue-cultured seedlings have 3-4 true leaves, move them to room temperature for 1 day, then remove the bottle cap and harden the seedlings for 1 day, wash the residual medium at the base, soak the tissue-cultured seedlings in 0.5% potassium permanganate solution for 10 minutes, The base was directly transplanted without any concentration of hormone treatment. Wrap the lower part of the Phalaenopsis seedlings (about 1.5 cm in length) with moss soaked in 0.5% potassium permanganate for 4 hours and dehydrated by a washing machine, and then put them into a 1.5-inch seedling cup. The...
Embodiment 2
[0024] 1) Experiment specific operation:
[0025] From July to October 2013, the experimental operation was carried out according to the dates in Table 2.
[0026] The date arrangement of the concrete operation of table 2 present embodiment
[0027]
[0028]
[0029] The seedlings of the three varieties Dajiao, Wenjingangang, and Liulin Black Rose were cultivated for 15-20 days. When the tissue-cultured seedlings have 3-4 true leaves, move them to room temperature for 1 day, then remove the bottle cap and harden the seedlings for 1 day, wash the residual medium at the base, soak the tissue-cultured seedlings with 1000 times carbendazim for 10 minutes, and then use Soak the base of tissue cultured seedlings with NAA at a concentration of 0.0625 mg / L for 10 min. Then wrap the lower part of the tissue cultured seedlings with sphagnum moss soaked in 0.5% potassium permanganate for 4 hours and dehydrated in a washing machine. Wrap the lower part of the Phalaenopsis seedlin...
Embodiment 3
[0034] 1) Experiment specific operation:
[0035] From October 2013 to February 2014, experiments were carried out according to Table 4.
[0036] Table 4 The specific implementation date of this embodiment
[0037]
[0038]
[0039] Cultivate the seedlings of the three varieties of big pepper, Wenjingangang, and Liulin black rose for 15 days. When there are 3-4 true leaves, move them to room temperature for 1 day, then remove the bottle cap and harden the seedlings for 1 day, and wash the medium After the seedlings were soaked in 0.5% potassium permanganate solution for 5 minutes, the base of the tissue cultured seedlings was soaked in IBA with a concentration of 50, 100, 150, and 200 mg / L for 10 minutes. Then wrap the lower part of the tissue cultured seedlings with sphagnum moss soaked in 0.5% potassium permanganate for 5 hours and dehydrated in a washing machine. Wrap the lower part of the Phalaenopsis seedlings (about 1.5cm in length), and put them into a 1.5-inch ...
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