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Preparation method of a new animal model of asthma

An animal model and asthma technology, applied in the field of medicine, can solve the problems of poor reactivity, invisible, weakened bronchospasm, etc., and achieve the effect of good animal reactivity and simple operation.

Active Publication Date: 2017-06-16
INST OF LAB ANIMAL SCI CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The first is a classic model, which measures airway resistance after anesthetizing animals sensitized to allergens. However, since animals are no longer normal animals after anesthesia, their reactivity is not good, and generally only the early changes of asthma and asthma can be observed. It is difficult to observe the delayed phase in the late stage of asthma attack, and the animal is under anesthesia, the vagus nerve is inhibited, and the bronchospasm will be weakened, which cannot truly reflect the degree of bronchospasm
The second is the airway hyperresponsiveness model. This model is mainly aimed at the airway inflammation after an asthma attack. The airway is overly sensitive under inflammatory conditions to study, although it can be used in animals under non-anesthesia Experiments are carried out, but the airway hyperresponsiveness model is observed after the asthma attack, and the asthma attack process has been missed, and non-asthmatic diseases such as bronchitis, chronic obstructive pulmonary disease, etc. can also have airway hyperresponsiveness, so the airway hyperresponsiveness Dow hyperresponsiveness model is not specific for asthma
No such report to date in rodent asthma models

Method used

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  • Preparation method of a new animal model of asthma
  • Preparation method of a new animal model of asthma
  • Preparation method of a new animal model of asthma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Embodiment 1, the preparation of asthma animal model

[0048] Experimental materials: egg protein (purchased from Sigma-Aldrich Company of the United States), dry powder of aluminum hydroxide (purchased from Sigma-Aldrich Company of the United States), normal saline, Norwegian brown rats of 6-8 weeks (purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd. limited company)

[0049] Preparation:

[0050] 1. Mix 25mg of egg protein and 250mg of aluminum hydroxide dry powder and add 1mL of normal saline to make a sensitization solution;

[0051] 2. Take 0.5 mg of egg protein and add 10 mL of normal saline to make 10 mL of 5% egg protein-normal saline solution;

[0052] 3. Take Norway brown rats of 6 to 8 weeks old, and inject the sensitizing solution subcutaneously at two points, 0.2ml per point;

[0053] 4. On the 5th day, repeatedly inject the sensitizing solution subcutaneously in rats at two points, 0.2ml per point;

[0054] 5. On the 37th day...

Embodiment 2

[0057] Embodiment 2, animal model identification of asthma

[0058] Animals were given blood samples before ovalbumin sensitization injection and on the 7th, 14th, 21st, 28th and 35th day after the injection to detect specific IgE. The results showed that IgE appeared from day 14 and peaked at day 28.

[0059] 24 hours after the asthma attack was provoked, the lungs of the animals were taken out and observed under a HE staining optical microscope. Eosinophilic leukocyte infiltration, increased mucus secretion and focal hemorrhage were seen in the bronchi and alveolar cavities, which were consistent with the inflammatory changes of allergic bronchial asthma attack. see results image 3 , 4 .

Embodiment 3

[0060] Embodiment 3, the method for screening the medicine for the treatment of asthma

[0061] Experimental materials: 6 to 8-week-old inbred Norwegian brown rat (purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd.), dexamethasone sodium phosphate injection (purchased from: Tianjin Jinyao Group Hubei Tianyao Pharmaceutical Co., Ltd. company).

[0062] The inbred Norwegian brown rats of 6 to 8 weeks were divided into three groups: normal saline control group, normal control group, and test drug group. According to the method of Example 1, the normal saline control group and the test drug group were sensitized with egg protein injection. , asthma was stimulated by nebulization of ovalbumin liquid; the normal control group was injected with normal saline and stimulated by nebulized inhalation of normal saline, and then all were recorded by non-invasive whole body plethysmography detector for 16-20 hours. The results of the drug group to be tested are sh...

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Abstract

The invention belongs to the field of medicine, and in particular relates to a preparation method of a novel animal model of asthma. The preparation method of the asthma animal model of the invention is simple to operate and does not require anesthesia. The asthma animal model obtained by the preparation method of the present invention has good animal responsiveness, is suitable for studying the whole reaction process of asthma attack including the immediate phase and the delayed phase of asthma, and can be used for screening and discovering new drugs for treating asthma, etc. Research.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to a method for preparing a novel animal model of asthma and a method for screening drugs by using the model. Background technique [0002] Asthma refers to an allergic and inflammatory disease of common airways, and its full name is allergic asthma or allergic asthma. The main features are variable and recurrent symptoms, reversible airflow obstruction, and bronchospasm. Common symptoms are wheezing, coughing, chest tightness, tightness, and shortness of breath. If the condition is not effectively controlled for a long time, airway remodeling may occur, causing irreversible airway narrowing. The incidence of asthma worldwide increased substantially between the 1960s and 2008 and has been recognized as a major public health problem since the 1970s. Since the 1990s, the incidence of asthma has stabilized in developed countries, while it has increased rapidly in developing count...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/38A61K49/00A61K33/08
Inventor 张星东秦川陶婧婧
Owner INST OF LAB ANIMAL SCI CHINESE ACAD OF MEDICAL SCI
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