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A biocontrol strain, preparation and application thereof capable of preventing grape downy mildew and capsicum blight

A technology for grape downy mildew and pepper blight, applied in the direction of application, biocide, fungicide, etc., can solve problems that threaten food safety, fruit and environmental pollution, etc., achieve good control effect, no toxic side effects, and broad application prospects Effect

Active Publication Date: 2017-10-17
LIAONING ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] At present, the control of grape downy mildew and pepper blight only relies on chemical pesticides, which not only cause serious pollution to fruits and the environment, but also threaten food safety.
The use of biocontrol fungicides to control grape downy mildew and pepper blight has few research reports, and there is no registered product

Method used

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  • A biocontrol strain, preparation and application thereof capable of preventing grape downy mildew and capsicum blight
  • A biocontrol strain, preparation and application thereof capable of preventing grape downy mildew and capsicum blight
  • A biocontrol strain, preparation and application thereof capable of preventing grape downy mildew and capsicum blight

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Experimental program
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Embodiment 1

[0027] 1. Strain preparation. Strain Streptomyces niger PY-1 was activated and cultured on PSA medium at a culture temperature of 25-30°C and cultured in the dark for 4 days. PSA medium composition: 200g potato, 20g sucrose, 20g agar, 1000mL distilled water.

[0028] 2. Preparation of biological control agents. Liquid medium ingredients: 200g potato, 20g sucrose, 1000mL distilled water. The filling volume is 200mL (500mL Erlenmeyer flask), and the inoculum volume is 1×10 9 cfu, fermentation temperature 27°C, 150r / min shaking fermentation culture for 5 days, the fermentation liquid was centrifuged at 12000r / min for 30min, and the supernatant was filtered through a 0.45μm filter membrane to prepare a stock solution of biocontrol agents capable of preventing grape downy mildew and pepper blight , diluted 300 times with water to carry out indoor control experiments.

[0029] Indoor and in vitro control effect evaluation of grape downy mildew.

[0030] The detached leaves we...

Embodiment 2

[0041] Evaluation of the control effect of indoor flat panel on the control of Phytophthora capsici by law enforcement.

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Abstract

A biocontrol strain, preparation and application capable of preventing grape downy mildew and capsicum blight, characterized in that the biocontrol strain is derived from Streptomyces diablos PY-1 strain from the surface soil of a vineyard, and is preserved in China Microorganisms General Microbiology Center of the Preservation Management Committee, the preservation number is CGMCC.No: 7826; the active ingredient in the biocontrol agent is the secondary metabolite of Streptomyces diablos PY‑1, wherein Streptomyces diablos PY‑1 was inoculated in a 500mL Erlenmeyer flask Cultivate in the 200mL PSA culture medium in the inside for 5 days, centrifuge the fermented liquid for 30min, and filter the supernatant to obtain the stock solution. Blight, eggplant root rot, tomato powdery mildew, tomato late blight, cucumber anthracnose, corn ear rot, sorghum Curvularia leaf spot, and wheat root rot also have certain inhibitory effects.

Description

technical field [0001] The invention relates to a biocontrol strain, a preparation and its application, in particular to a biocontrol strain capable of preventing and treating grape downy mildew and capsicum blight, a preparation and its application, belonging to the technology of biocontrol preparations made from beneficial biocontrol strains field. Background technique [0002] Grape downy mildew is caused by grape downy mildew ( Plasmopara viticola (Berk. and Curt.) Berl. and de Toni) caused by fungal disease, is a worldwide ancient disease, all over the grape producing areas at home and abroad, is one of the main grape leaf diseases. The disease was first discovered on wild grapes in eastern North America. In 1870, Europe introduced American phylloxera-resistant rootstocks, and grape downy mildew entered Europe. A few years later, downy mildew first occurred on a large scale in southern France, and then spread throughout Europe, becoming the primary disease of local gr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00A01G7/06C12R1/465
CPCA01G13/00A01N63/10C12N1/205C12R2001/465
Inventor 梁春浩刘长远白元俊关天舒王辉刘丽于舒怡黄玉茜赵达臧超群李柏宏
Owner LIAONING ACAD OF AGRI SCI