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Synthetic method of test object for determining astragaloside content in radix astragali medicinal material

A technology of astragaloside IV and a synthesis method, which is applied in the field of test substance synthesis for determination of astragaloside IV content in astragalus medicinal materials, can solve the problems of inability to reflect the content of astragaloside I glycoside derivatives, and achieve the effect of accurate and effective results

Inactive Publication Date: 2015-03-25
李月梅
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the process of our research, we also found that they were all extracted with methanol first, and then treated with alkali. The conversion results of this method could not reflect the content of all the derivatives of Astragalus membranaceus in Astragalus membranaceus, and some of them were soluble in dilute Alcoholic but slightly soluble astragaloside derivatives in methanol were not extracted

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0007] Example 1: Take about 1 g of Astragalus membranaceus powder, weigh it accurately, put it in a stoppered Erlenmeyer flask, add 5 mL of ammonia test solution, shake to make it fully wet, then add 50 mL of n-butanol, seal it tightly, and process it ultrasonically. Power 600 W, 33 kHz, 15 min, let stand to separate layers, pour out the n-butanol solution in the upper layer, add 50 mL of n-butanol to the lower layer, and treat once more in the same way, combine the n-butanol solution twice, filter, and the filtrate Wash with water twice, 30 mL each time, discard the water, evaporate the n-butanol solution to dryness, dissolve the residue with methanol and set the volume to 10 mL to obtain the test solution.

Embodiment 2

[0008] Example 2: Take about 1 g of Astragalus membranaceus powder, weigh it accurately, put it in a stoppered Erlenmeyer flask, add 6 mL of ammonia test solution, shake to make it fully wet, then add 60 mL of n-butanol, seal it tightly, and ultrasonically treat it. Power 600 W, 33 kHz, 15 min, let stand to separate layers, pour out the n-butanol solution in the upper layer, add 50 mL of n-butanol to the lower layer, and treat once more in the same way, combine the n-butanol solution twice, filter, and the filtrate Wash with water twice, 30 mL each time, discard the water, evaporate the n-butanol solution to dryness, dissolve the residue with methanol and set the volume to 10 mL to obtain the test solution.

Embodiment 3

[0009] Example 3: Take about 1 g of Astragalus membranaceus powder, accurately weigh it, put it in a stoppered Erlenmeyer flask, add 7 mL of ammonia test solution, shake it to make it fully wet, then add 70 mL of n-butanol, seal it tightly, and process it ultrasonically. Power 600 W, 33 kHz, 15 min, let stand to separate layers, pour out the n-butanol solution in the upper layer, add 50 mL of n-butanol to the lower layer, and treat once more in the same way, combine the n-butanol solution twice, filter, and the filtrate Wash with water twice, 30 mL each time, discard the water, evaporate the n-butanol solution to dryness, dissolve the residue with methanol and set the volume to 10 mL to obtain the test solution.

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Abstract

A synthetic method of a test object for determining astragaloside content in a radix astragali medicinal material belongs to the technical field of traditional Chinese medicine component determination, and in particular, the invention relates to a synthetic method of a test object for determining astragaloside content in a radix astragali medicinal material. The invention provides a preparation method of a control solution for determining stilbene content in chinaroot greenbrier. The method of the invention comprises: taking about 1 g of radix astragali medicinal material medium powder, weighing precisely, putting the medium powder in a conical flask with a plug, adding 5-10 mL of an ammonia test solution, shaking for complete wetting, adding 50-100 mL of n-butanol, sealing with the plug, performing ultrasonic treatment at a power of 600 W and a frequency of 33 kHz for 15 min, allowing the solution to stand for layering, pouring out the n-butanol liquid in the upper layer, adding 50 mL of n-butanol into the lower layer, treating the solution once in the same manner, combining the two n-butanol liquids, filtering, washing the filtrate with water twice, 30 mL of water each time, discarding the water, evaporating the n-butanol liquid, dissolving the residue with methanol, and adding methanol to obtain a volume of 10 mL, so as to obtain the test object solution.

Description

technical field [0001] The invention belongs to the technical field of determination of traditional Chinese medicine components, in particular, the invention relates to a method for synthesizing a test product used for determining the content of astragaloside IV in astragali medicinal materials. Background technique [0002] Astragalus is a commonly used traditional Chinese medicine, which has the effects of invigorating qi and raising yang, benefiting health and solidifying the surface, diuresis and swelling, and promoting granulation of sores. The main chemical components in Astragalus include saponins, flavonoids and polysaccharides. Studies have confirmed that astragaloside IV in Astragalus membranaceus is the main active ingredient for the positive inotropic effect of the heart, and it is also one of the effective ingredients for lowering blood pressure. During the research process, we found that the content of astragaloside IV in the original form in Astragaloside is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28
Inventor 李月梅董开宇
Owner 李月梅
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