A one-component enzyme substrate chromogenic solution

Abstract

The invention discloses a single-component enzyme substrate color development solution. The color development solution per 1000 mL comprises the following components: 7-10 g of citric acid, 16-20 g of sodium hydrogen phosphate, 0.1-0.5 g of perboric acid soda, 20-30 ml of glycerol, 5-40 mg of enzyme substrates and 10-30 ml of alcohol. According to the single-component enzyme substrate color development solution, conventional hydrogen peroxide and urea peroxide are replaced with perboric acid soda which has a better oxidizing property compared with hydrogen peroxide and urea peroxide, so that the color development effect of the single-component enzyme substrate color development solution is improved; meanwhile, an operation step that a solution A and a solution B need to be mixed before use when the single-component enzyme substrate color development solution is used is eliminated, so that the operating process is simplified, the operation time is shortened, and the efficiency is improved; influence on the color development effect of a mixed color developing agent, caused by difference between the solution A and the solution B in different batches, is avoided; change of the concentration of effective color development components of the sing-component enzyme substrate color development solution is avoided, so that the sensitivity of the sing-component enzyme substrate color development solution is improved.

Description

technical field [0001] The invention relates to the field of enzyme substrate chromogenic solution, in particular to a one-component enzyme substrate chromogenic solution. Background technique [0002] The enzyme substrate is TMB, which is used for ELISA reaction. The substrate produces a soluble light blue end product that can be read on a spectrophotometer at 370 or 635 nm. It is suitable for quantitative and qualitative analysis in the chromogenic stage of ELISA. The principle of the test is: when the enzyme labeled on the antibody or antigen reacts with the enzyme substrate, hydrogen peroxide, etc. in the chromogenic solution, the solution changes from colorless to yellow after adding the stop solution, and the depth of yellow reflects the combination of antigen and antibody in the ELISA system How much, so as to judge the amount of a certain antigen or antibody. Measure the absorbance value with a microplate reader to judge the degree of immune reaction. [0003] Mo...

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Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a single-component enzyme substrate chromogenic solution to overcome the problems of troublesome operation and low sensitivity of the existing chromogenic solution

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