In-situ high flux culture apparatus for sea microbe

A high-throughput technology for marine microorganisms, applied in enzymology/microbiology devices, biochemical cleaning devices, methods of supporting/immobilizing microorganisms, etc., can solve cumbersome operations, high equipment requirements, and the proportion of marine microorganisms is less than 1%, etc. problems, to reduce damage and avoid pollution

Inactive Publication Date: 2015-04-08
OCEAN UNIV OF CHINA
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  • Summary
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the lack of effective cultivation methods, our understanding of its diversity, physiological and ecological functions and the development of its functions have been hindered.
So far, less than 1% of the total number of marine microorganisms cultured and isolated by traditional culture methods
[0005] At present, the combination of chromatographic column and peristaltic pump is widely used. The peristaltic pump can maintain the stability of the culture environment, and the chromatographic column can divide the culture environment into partitions, so that large-scale parallel isolation culture can be carried out; the principle of this method is simple. , but the operation is cumbersome and requires high equipment
The high-throughput culture method has more or less shortcomings, how to improve its culture efficiency is the difficulty of microbial culture

Method used

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  • In-situ high flux culture apparatus for sea microbe
  • In-situ high flux culture apparatus for sea microbe
  • In-situ high flux culture apparatus for sea microbe

Examples

Experimental program
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Effect test

Embodiment 1

[0044] When applied to the laboratory to simulate the in-situ environment, the use of figure 1 and figure 2 The method for cultivating and screening marine microorganisms with the device comprises the following steps;

[0045] (1) Preparation of marine microbial microspheres: the concentrated seawater (200:1) was mixed with the microsphere preparation solution, and calcium alginate microspheres with a particle size of about 50 microns embedded with marine microorganisms were prepared by the coaxial air flow method. The ratio of the prepared microspheres to the microbes embedded in the microspheres is 10:1;

[0046] (2) After sterilizing the culture chambers, add 80 mg of microspheres prepared in step 1 to each chamber, and cover with an independent top cover connected to the conduit 12 for sealing;

[0047] (3) Add recycled seawater obtained in the field to the constant temperature oscillator, place the whole device in a constant temperature oscillator with a temperature of...

Embodiment 2

[0051] When applied to in situ conditions in the ocean, the use of image 3 and Figure 4 The method for cultivating and screening marine microorganisms with the device comprises the following steps;

[0052] (1) Prepare marine microorganism microspheres: the concentrated seawater (200:1) is mixed with the microsphere preparation solution, and calcium alginate microspheres with a particle diameter of about 50 microns embedded with marine microorganisms are prepared by the coaxial air flow method;

[0053] (2) After the culture chamber is sterilized, add 80 mg of microspheres prepared in step 1 to each well, and cover with a translucent top plate to seal;

[0054] (3) Suspended at a depth of 40 meters below the sea surface in the South China Sea with the assistance of other floating devices, the seawater enters the seawater tank through the sieve holes of the open bottom plate and communicates with the cultivation chamber through the filter membrane;

[0055] (4) After the cu...

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Abstract

The invention discloses an in-situ high flux culture apparatus for sea microbe, which comprises a seawater cabin and an independent culture chamber; the seawater cabin comprises a base plate fixed by bolts, a liquid storage layer, a partition plate and a culture layer from down to up, a reservoir is opened at intermediate part of the liquid storage layer, the circular holes with same size and relative position are opened on the partition plate and the culture layer; a layer of filter membrane is arranged between the partition plate and the culture layer, and the culture chamber is positioned in the circular hole on the culture layer and positioned on the filter membrane. When the apparatus is used for simulating in-situ environment in a laboratory, the apparatus is placed in a constant temperature oscillator, the base plate is a sealing base plate, the culture chamber is sealed by using an independent top cover connected to a conduit; when the apparatus is used for in-situ culture, the base plate is the base plate with sieve apertures, and the top of the culture chamber is sealed with a light transmission top board. The apparatus has the characteristics of integral disinfection, high pressure resistance, temperature difference resistance, and bump resistance. The apparatus can keep stable in-situ culture environment in laboratory and sea environment, and damage on the microbe due to environment change can be reduced.

Description

technical field [0001] The invention relates to a marine microorganism cultivation device, in particular to a device for in-situ high-throughput cultivation of marine microorganisms, which belongs to the field of microorganism cultivation devices. Background technique [0002] There are extremely rich microbial resources in the ocean, which are more and more closely related to human production and life and environmental protection. However, due to the lack of effective cultivation methods, we have hindered our understanding of its diversity, physiological and ecological functions and the development of its functions. So far, less than 1% of marine microorganisms have been cultured and isolated by traditional culture methods. [0003] High throughput technology is a new type of technology that appeared in the 1980s. It is to cultivate, identify and screen micro-quantified experimental systems at the molecular or cellular level. High-throughput technology is widely used in d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12M1/12
CPCC12M23/04C12M23/12C12M23/34C12M25/04
Inventor 刘晨光魏文浩张晓华李诚博张增虎
Owner OCEAN UNIV OF CHINA
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