A new LC-MS/MS-based method for the determination of arginine

Abstract

The invention discloses a novel arginine determination method based on LC-MS / MS. According to the method, specific derivatization reagents and LC-MS / MS are utilized for accurately and quantitatively detecting derivative arginine product, with the adoption of the method, the mass spectrum response value is improved by more than 10 times, the derivatization reaction is rapid, is complete within 10 minutes, and nontoxic and efficient effects are achieved; the product is well preserved on a universal analytical column C18, so that analysis is facilitated, the residual PEITC subjected to reaction is easily volatilized to be removed, moreover, a carbon chain is long, so that the analytical column is not damaged, the analysis range is four orders of magnitude within 100pg / mL-1microg / mL.

Description

technical field [0001] The present invention relates to a method based on the use of a specific derivatization reagent, in particular to a method for determining the concentration of arginine in plasma by LC-MS / MS after derivatization of arginine acid. Background technique [0002] Arginine (L-Arg) is one of the most functional amino acids in animal cells. It participates in the process of synthesizing cytoplasmic protein and nuclear protein, and plays an important role in various physiological processes such as cell division, protein synthesis and hormone secretion. [0003] Arginine is also a starting material for polyamine synthesis. Polyamine is an important biological regulatory substance, which is related to the biological metabolism of DNA, RNA and protein, and plays a key regulatory role in the process of cell growth cycle; it participates in the T cell immune response induced by mitogens, and plays a key role in regulating the central nervous system. It plays a ke...

AI Technical Summary

Problems solved by technology

However, its structure is too short, and trace residues will reduce the service life of ordinary C18 analytical columns, which is not convenient for the processing and analysis of a large number of samples

[0009] Fluorenylmethyl chloroformate, AQC and OPA all use fluorescence detection, but the reaction conditions are harsh, and there is also the problem of narrow analytical quantitative range, and when hydroxyproline and hydroxylysine exist in the sample, the reaction by-products will be serious Interfering with quantification, so when analyzing plasma samples, it is necessary to carefully consider the influence of the matrix; dansyl chloride derivative products have greater interference, and the peak shape is poor, which is not conducive to accurate quantification; DNFB derivative reagents are more toxic; excessive derivative reagents are harmful to the analysis. certain influence