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Method for preparing industrial alcohol by fermenting cane molasses through lactobacillus casei

A technology of Lactobacillus casei and sugarcane molasses, which is applied in the recycling of industrial waste and in the field of microorganisms to achieve high output rates

Inactive Publication Date: 2015-04-22
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But at present there is no effective method to produce ethanol by Lactobacillus casei and greatly increase the ethanol production

Method used

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  • Method for preparing industrial alcohol by fermenting cane molasses through lactobacillus casei

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specific Embodiment approach 1

[0023] The specific embodiment one: a kind of method utilizing Lactobacillus casei to ferment sugarcane molasses of the present embodiment to prepare industrial ethanol, it is carried out according to the following steps:

[0024] 1. Pick Lactobacillus casei from the cryopreserved glycerol tube and insert it into the MRS liquid medium, and cultivate it at 37°C for 24 hours; wherein, the pH of the MRS liquid medium is 6.0;

[0025] 2. For the strains cultivated in step 1, the concentration is 1×10 8 Insert CFU / mL into sterilized sugarcane molasses solution, and incubate at 37°C for 24-30 hours;

[0026] 3. Fill the sugarcane molasses fermentation liquid into the fermenter, seal all the inlets and outlets, sterilize and cool, and set aside;

[0027] 4. For the strains cultivated in step 2, the concentration is 1×10 8 Connect CFU / mL to the fermenter in step 3 for fermentation and culture, set up an automatic pH constant system to keep the pH value constant at 5.95-6.05, set the...

specific Embodiment approach 2

[0030] Embodiment 2: This embodiment differs from Embodiment 1 in that: NaOH solution and HCl solution are used to adjust the pH value of the molasses fermentation liquid to 6.0. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0031] Embodiment 3: The difference between this embodiment and Embodiment 1 is that the lye used to keep the pH value constant is NaOH solution, and the acid solution is HCl solution. Others are the same as in the first embodiment.

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Abstract

The invention provides a method for preparing industrial alcohol by fermenting cane molasses through lactobacillus casei. The method for preparing the industrial alcohol by fermenting the cane molasses through the lactobacillus casei includes the steps that the Brix degree of the cane molasses is reduced to 5 degrees through dilution, the lactobacillus casei is added for necessary nitrogen source growth, and the pH value is adjusted to 6.0; a fermentation tank is filled with the mixture and sealed and the lactobacillus casei with the concentration being 1*108 CFU / mL is added after the mixture is sterilized and cooled, wherein the constant pH value of the fermentation tank is 6.0 and the number of turns of a magnetic stirrer is set to be 100 rpm, the tank is inflated with inert gas, an anaerobic fermentation environment is maintained, and fermentation is conducted at the temperature of 37 DEG C for 48 h. By the adoption of the method, the fermentation rate can be increased, the use rate of carbohydrate in the molasses is increased, and pollution caused by molasses alcoholic fermentation waste liquid is reduced.

Description

technical field [0001] The invention belongs to the field of industrial waste recycling and the field of microorganisms. Specifically relates to a method for preparing industrial ethanol by using Lactobacillus casei to ferment sugarcane molasses. Background technique [0002] Sugarcane molasses is a dark brown, viscous by-product separated from sugarcane as raw material in sugar factories, after pressing, clarification and purification of cane juice, filtration, evaporation, concentration, and crystallization of sugar, commonly known as orange juice. Its yield is 2.5% to 3.0% of the raw material sugarcane. Its total sugar content is about 50%, and its Brix is ​​greater than 60°Bx. Sugarcane molasses contains water, sugar, non-sugar organic matter, protein, vitamins and trace elements and other available components. The main sugars are sucrose, glucose and fructose. Because of its large proportion of sugar, there are sufficient carbon sources for microorganisms to convert...

Claims

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Application Information

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IPC IPC(8): C12P7/06C12R1/245
CPCC12P7/065Y02E50/10Y02P20/54
Inventor 王松张柏林赵宏飞周方
Owner BEIJING FORESTRY UNIVERSITY
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