Method for quickly separating Synechocystis cells and mineralization products thereof

Abstract

The invention discloses a method for quickly separating Synechocystis cells and mineralization products thereof, which comprises the following steps: putting a certain amount of Synechocystis cell culture solution in a culture bottle, performing ultrasonic treatment, and tilting the culture bottle, wherein a certain amount of mineral precipitate can aggregate at the bottom of the culture bottle; moving the mineral precipitate into a centrifuge tube, centrifuging, and collecting a precipitate I; adding a mixed solution containing diethyl ether, acetone and anhydrous ethanol into the precipitate I, and repeatedly sucking and injecting to enable the precipitate I to suspend, thus obtaining a suspension I; performing ultrasonic treatment on the suspension I, and then collecting a precipitate II; adding a mixed solution of methanol and anhydrous ethanol into the precipitate II, and repeatedly sucking and injecting to enable the precipitate II to suspend, thus obtaining a suspension II; performing ultrasonic treatment on the suspension II, and collecting a precipitate III; and adding anhydrous ethanol into the precipitate III, and washing for multiple times to obtain the mineralization products. The mineralization products separated by the invention have unbroken surface topography, thereby being extremely beneficial to analyze the surface structure thereof through a scanning electron microscope.

Description

technical field [0001] The invention belongs to the technical field of bioenergy, and in particular relates to a method for separating Synechocystis cells and their mineralization products. Background technique [0002] Geochemical models and laboratory experiments have confirmed that the specific metabolic reactions of microorganisms play a very important role in the biomineralization of microbial carbonates, so researchers have started work to explore the various mechanisms that form microbial carbonates. Physicochemical mechanism. Cyanobacteria are recognized as a prokaryotic organism that can undergo biomineralization. For example, Synechocystis PCC6803, which is often used in the laboratory, can induce calcite biomineralization in BG11 liquid medium containing different calcium ion concentrations. The focus of researchers' research is mainly on the mechanism of biomineralization. This part of the work includes the analysis of changes in the micromorphology and structur...

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Problems solved by technology

The focus of researchers' research is mainly on the mechanism of biomineralization. This part of the work includes the analysis of changes in the micromorphology and structure of the mineral surface. However, in this part of the work, a problem has been encountered. During the biomineralization process, it is difficult to observe the surface micromorphology of minerals in detail because Synechocystis closely adheres to the mineral surface

[0003] At present, there are few methods for separating biominerals induced by microorganisms. Conventional treatment techniques in the prior art have, such as using 30% hydrogen peroxide to treat and remove Synechocystis and other substances adhered to the mineral surface, but the disadvantage of this treatment technique is The surface structure of minerals will be destroyed, so it is necessary to study a treatment method that can protect the surface structure of minerals

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