Method for extracting steroid hormone from excrement of forest musk deer

A steroid hormone and extract technology, applied in the biological field, can solve the problems of negative growth and slow growth rate of captive musk deer populations

Inactive Publication Date: 2015-04-29
BEIJING FORESTRY UNIVERSITY
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  • Application Information

AI Technical Summary

Problems solved by technology

Although my country has started the captivity of forest musk deer for more than 50 years, the growth rat

Method used

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  • Method for extracting steroid hormone from excrement of forest musk deer
  • Method for extracting steroid hormone from excrement of forest musk deer
  • Method for extracting steroid hormone from excrement of forest musk deer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1, the extraction of cortisol in forest musk deer feces

[0031] 1. Extraction of cortisol in feces of female musk deer

[0032] Selected six female musk deer from the Pien Tze Huang forest musk deer farm in Miyaluo, Sichuan, and named them respectively as the female musk deer A, the female forest musk deer B, the female forest musk deer C, the female forest musk deer D, the female forest musk deer E and the female forest musk deer already.

[0033] 1.1 Collection of feces from female musk deer

[0034] The fresh feces of the above 6 female forest musk deer were collected for 4 consecutive days, and each female forest musk deer was collected once a day, 30g each time. Put the fresh feces collected each time into a ziplock bag (one ziplock bag for each feces collected by each female forest musk deer), and seal the seal with the air removed to obtain the feces of the first day of the female forest musk deer and the second day of the female forest musk deer re...

Embodiment 2

[0085] Embodiment 2, the extraction of estradiol in the feces of female forest musk deer

[0086] According to the following method, measure the content of estradiol in the female forest musk deer 100M extract of embodiment 1, the female forest musk deer 90M extract, the female forest musk deer 80M extract and the female forest musk deer 60M extract. content:

[0087] Adopt chemiluminescence method to measure the content of estradiol in the above-mentioned leaching solution, analyzer uses the chemiluminescence instrument of 200s model that Xiamen Tian Zhongda produces; Adopt estradiol (E2) quantitative detection kit (chemiluminescence method) (estradiol) The diol (E2) quantitative detection kit (chemiluminescence method) is a product of Beijing North Institute of Biotechnology. The main components of the kit are shown in Table 6, and the main performance indicators are as follows:

[0088] a. Measurement range: 25-1000pg / ml

[0089] b. Minimum detectable amount: ≤13pg / ml

...

Embodiment 3

[0117] Embodiment 3, the extraction of testosterone in male forest musk deer feces

[0118] According to the following method, measure the content of testosterone in the male forest musk deer 100M extract, the male forest musk deer 90M extract, the male forest musk deer 80M extract and the male forest musk deer 60M extract of embodiment 1:

[0119] The content of testosterone in the above extracts was determined by enzyme-linked immunosorbent assay. The enzyme label analyzer is ThermoMultiscan FC type, adopts testosterone quantitative assay kit (enzyme-linked immunoassay) (this kit is the product of Beijing North Institute of Biotechnology), the main components of the kit are shown in Table 8 and Table 9, testosterone The main technical parameters of the quantitative assay kit (ELISA) are as follows:

[0120] a. Sensitivity: ≤0.05ng / ml

[0121] b. Precision: Coefficient of Variation CV<15﹪

[0122] c. Specificity: no cross-reaction with progesterone, estradiol, DHT

[0123...

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Abstract

The invention discloses a method for extracting steroid hormone from excrement of forest musk deer. The method comprises the following step of leaching animal excrement by virtue of extraction liquid so as to obtain leaching liquid containing the steroid hormone, wherein the extraction liquid is an ethanol water solution, a methanol water solution, ethanol or methanol; the concentration of volume percent of ethanol in the ethanol water solution is more than or equal to 60% and less than 100%; the concentration of volume percent of methanol in the methanol water solution is more than or equal to 60% and less than 100%; the steroid hormone is one or two or all of cortisol, estradiol and testosterone. An experiment proves that the steroid hormone is extracted from the excrement of the forest musk deer by virtue of the extraction method, the optimal extraction liquid is 90E (10% ethanol water solution).

Description

technical field [0001] The invention relates to a method for extracting steroid hormones in musk deer feces in the field of biotechnology. Background technique [0002] Wood musk deer is a small forest-dwelling ruminant ungulate, and it is a precious medicinal animal native to Asia. Although my country has started captive breeding of forest musk deer for more than 50 years, the growth rate of the captive musk deer population has been slow, and even negative growth has occurred in some years. The main reasons are as follows: 1. The female deer musk deer raised in artificial captivity has a relatively high rate of empty breasts after the breeding period; 2. The deer musk deer animals are naturally timid and vigilant, quite vigilant and strong in stress. Cortisol is a steroid hormone that can directly reflect the physiological stress of animals; both estradiol and testosterone are steroid hormones, and these two hormones can well reflect the reproductive physiological state of...

Claims

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Application Information

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IPC IPC(8): C07J5/00C07J1/00C07J75/00
CPCC07J1/0022C07J1/007C07J5/0053
Inventor 王毅花刘树强周俊彤葛兴芳胡德夫
Owner BEIJING FORESTRY UNIVERSITY
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