Method for open-type fast cultivation of oil-bearing microbes

An open and microbial technology, which is applied in the field of increasing the oil content of oil-containing microbial cells and cultivating oil-containing microorganisms in an open manner, can solve the problems of low oil accumulation level, different requirements for wall breaking, and poor results, so as to increase the intensity of photosynthesis and improve Cumulative effect, effect of efficient growth process

Active Publication Date: 2015-04-29
CHINA PETROLEUM & CHEM CORP +1
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Problems solved by technology

However, since the mixed culture of yeast and algae is a heterotrophic culture, the level of oil accumulation in the cells is low; and the two kinds of cells harvest a...

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  • Method for open-type fast cultivation of oil-bearing microbes
  • Method for open-type fast cultivation of oil-bearing microbes

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Embodiment Construction

[0028]The open method for cultivating oil-containing microorganisms of the present invention specifically includes the following content: before the semi-mixed culture, two kinds of oil-containing microorganisms are separately cultured in seed liquid, and a stirring bioreactor is used for culturing oil-containing yeast seed liquid, and a photobioreactor is used for culturing the oily yeast seed liquid. For oil-containing microalgae seed liquid cultivation, the stirred bioreactor includes oleaginous yeast and oleaginous yeast fermentation medium, and the photobioreactor includes oleaginous microalgae and oleaginous microalgae medium. After the seed liquid culture reaches the logarithmic growth phase, the oil-containing yeast is inoculated into the lower area of ​​the open photobioreactor with a membrane separation device inside, and the oil-containing microalgae is inoculated into the upper area of ​​the photobioreactor through the pipeline. Both the inner wall of the reactor an...

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Abstract

The invention discloses a method for open-type fast cultivation of oil-bearing microbes. The method comprises the following steps: (1) cultivating an oil-bearing yeast seed liquid; (2) cultivating an oil-bearing microalgae seed liquid; and (3) throwing the oil-bearing yeast seed liquid and the oil-bearing microalgae seed liquid into an open-type photobioreactor with a membrane separation device laid therein for semi-mixed cultivation, wherein the membrane separation device divides the photobioreactor into an upper area and a lower area, oil-bearing yeast is cultivated in the lower area of the membrane separation device, oil-bearing microalgae is subjected to photosynthetic growth in the upper area of the membrane separation device, the culture medium compositions and the cultivation conditions of the upper area and the lower area are the same, organic carbon sources required for growth and metabolism of the oil-bearing yeast are supplemented in the cultivation process, and reflective membranes are arranged on the inner wall of the upper area of the photobioreactor and at the upper end of the membrane separation device. The method has the advantages of improving the cell yield of the oil-bearing microbes and the utilization ratio of inorganic carbon sources (CO2), simplifying a cultivation device, increasing the oil content of the microbes and the product total oil content and the like.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to an open method for cultivating oleaginous microorganisms, in particular to an open semi-mixed culture of oleaginous microalgae and oleaginous yeast to obtain a high yield of oil-containing microbial cells and simultaneously increase the oil content of oleaginous microbial cells content method. Background technique [0002] At present, countries around the world are facing the depletion of oil resources, CO 2 A series of climate problems and environmental pollution problems caused by emissions, and the development of renewable clean energy are known as research hotspots. Biodiesel derived from renewable resources such as microbial oil has high energy density, low sulfur content, sufficient combustion, and good lubricity. It is also renewable, environmentally friendly, easy to biodegrade, safe for storage and transportation, and anti-knock performance Good features, can be used as a su...

Claims

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Application Information

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IPC IPC(8): C12N1/18C12N1/16C12N1/12C12R1/865C12R1/645
CPCC12N1/12C12N1/16C12N1/18
Inventor 廖莎王领民师文静姚新武李晓姝张霖高大成乔凯
Owner CHINA PETROLEUM & CHEM CORP
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