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Two SNP markers in close linkage with cucumber powdery mildew resistance and application thereof

A technology for powdery mildew resistance and powdery mildew resistance, applied in the fields of genetic engineering and molecular biology, can solve the problems of weak powdery mildew resistance gene mapping research and no SNP markers, and achieve the effect of accelerating the breeding process

Active Publication Date: 2015-04-29
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although powdery mildew is one of the important diseases, the research on the mapping of powdery mildew resistance genes is relatively weak
The current reports mainly focus on the identification of powdery mildew resistance QTL, and there is no report on the use of SNP markers for assisted selection of cucumber breeding traits

Method used

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  • Two SNP markers in close linkage with cucumber powdery mildew resistance and application thereof
  • Two SNP markers in close linkage with cucumber powdery mildew resistance and application thereof
  • Two SNP markers in close linkage with cucumber powdery mildew resistance and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1. Construction of genetic segregation populations and identification of exchanged individual plants

[0024] 1. F 2 Group construction

[0025] D8 (female parent), high susceptibility to powdery mildew inbred line; quoted from the University of North Carolina, USA, the stem is thick, and when the internode grows to 12-14 nodes, the top is capped with a bunch of female flowers and stops growing, the leaves are dark green, and the fruit is short , thick flesh, dark green peel; JIN5-508 (male parent), highly resistant to powdery mildew; it is a creeping strain with tall plants and long internodes, which is derived from the domestic variety "Jinchun 5" (Tianjin Kerun Agricultural Technology Co., Ltd. The company) continuously selfed and separated for 8 generations, selected plants with high resistance to powdery mildew in each offspring, and self-crossed to the 8th generation to obtain a high-resistant cucumber powdery mildew and long-fruited inbred line. This implementa...

Embodiment 2

[0049] (1) Select 8 cucumber lines known to be resistant to powdery mildew, 4 of which are resistant to powdery mildew (R210, EP112, GY114, JD65), and 4 are susceptible to powdery mildew (LL017, CJ223, EP56, SD41);

[0050] (2) Extract the above-mentioned eight strains and parental genomic DNA respectively, use the dCAPS markers derived from Chr6SNP-pm01 and Chr6SNP-pm02 to perform PCR amplification, and then use AluI restriction endonuclease to digest the amplified Chr6SNP-pm01 primer pair The PCR product, using DdeI restriction endonuclease to digest the PCR product amplified by the Chr6SNP-pm02 primer pair;

[0051] (3) The results of agarose gel electrophoresis showed that the identification of disease resistance was consistent with the results of double enzyme digestion ( Figure 7 , Figure 8 ), the above two SNP markers can distinguish powdery mildew resistant from susceptible powdery mildew strains.

[0052]

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Abstract

The invention relates to molecular markers in close linkage with cucumber powdery mildew resistance in the technical field of genetic engineering and provides SNP markers in close linkage with cucumber powdery mildew resistance QTL. The SNP markers are respectively named as Chr6SNP-pm01 and Chr6SNP-pm02. The Chr6SNP-pm01 is positioned at the loci 4, 450 and 551 of the 6th chromosome, and the Chr6SNP-pm02 is positioned at the loci 6, 822 and 412 of the 6th chromosome. The SNP markers in close linkage with cucumber powdery mildew resistance are screened by utilizing variant groups and population genetics, the cucumber genotypes are detected by virtue of methods for sequencing and transforming into dCAPS markers, and the difference of the cucumber powdery mildew resistance can be judged according to the genotypes, so that the breeding progress of the cucumber powdery mildew resistance strain is accelerated. Meanwhile, the two SNP markers lay a foundation for cloning cucumber powdery mildew resistance genes.

Description

technical field [0001] The invention belongs to the fields of genetic engineering and molecular biology, and specifically relates to a SNP marker closely linked with cucumber powdery mildew resistance. Background technique [0002] Cucumber (Cucumis sativus L.) is an annual vine herbaceous plant of Cucurbitaceae (Cucumis) genus Cucumis. It is native to the warm and humid southern foothills of the Himalayas in northern India. It has been cultivated in my country for more than 2,000 years. As one of the ten most important vegetable crops in the world, cucumber is also one of the main vegetable crops in my country. According to the statistics of the World Food and Agriculture Organization (FAO), in 2012, the total cultivation area of ​​cucumbers in my country reached 1.1525 million hectares, and the annual output reached 48.0488 million tons, accounting for 54.63% and 73.77% of the world's total respectively. Cucumber production is threatened by many diseases, among which powd...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12Q1/68
Inventor 陈学好许学文徐瑞雪俞婷齐晓花徐强
Owner YANGZHOU UNIV
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