Breeding and molecular marker method of small-segment translocation line T1BL.1BS-2RL with wheat powdery mildew resistance

Abstract

The invention discloses a breeding method of wheat-Jingzhouheimai translocation line T1BL.1BS-2RL with wheat powdery mildew resistance. The translocation line bred according to the method is high in powdery mildew resistance in an adult-plant stage; the disclosed three molecular markers can track the translocation; and auxiliary selection and utilization of the translocation in wheat breeding are facilitated.

Description

1. Technical field [0001] This patent discloses a breeding method for wheat powdery mildew-resistant small segment translocation line T1BL.1BS-2RL, which belongs to the field of crop genetics and breeding and agricultural biotechnology. The selected translocation line has the effect of resisting powdery mildew. The published 3 This molecular marker can track the translocation, which is beneficial to the marker-assisted selection and utilization of the translocation line in wheat breeding, and provides new germplasm for breeding wheat varieties resistant to powdery mildew and broadening the genetic basis of cultivated wheat. 2. Background technology [0002] Wheat is the food crop with the largest planting area and the highest yield in the world. However, with the change of production environment and the selection and promotion of modern intensive varieties, the genetic diversity of cultivated wheat varieties is becoming narrower, which limits the high and stable yield of whe...

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Problems solved by technology

This patent uses 1600Rad dosage 60 C O -γ-ray irradiation DA2R-1 full-flowering tassels (2n=44) (see references: Li Aixia, Qi Zengjun, Pei Ziyou, et al. Breeding of common wheat Huixian red-Jingzhou rye heterochromatic system and its shuttle mosaic leaves Identification of disease resistance. Acta Crops, 2007, 33 (4): 639-645), taking pollen after radiation to pollinate common wheat Huixianhong (commonly known and public, local wheat variety in my country) for 3-5 days after emasculation, M 1 Seeds were self-fertilized after planting, and M 2 Identified, number 11LZQ60-8 M 2 The plant contains structural variations involving two rye chromosomes, one of which is a small fragment translocation chromosome and the other is an alocentromere chromosome, but its specific identity is not clear (see reference: Liu Zhenqian, Chromosomal variants of Jingzhou rye Induction and Identification. 2012, Master Thesis of Nanjing Agricultural University)

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