Rotary scanning real-time fluorescent quantitative PCR (Polymerase Chain Reaction) detection system

A detection system and rotary scanning technology, which is applied in the field of DNA fragment expansion and detection, can solve problems such as poor stability, high cost, and complex electromechanical structure, and achieve the effects of fast heating and cooling, long detection time, and high amplification efficiency

Inactive Publication Date: 2015-04-29
BEIJING UNIV OF TECH
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  • Summary
  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Two-dimensional motor-driven scanning, slow speed, complex electromechanical structure; cooling CCD, fast speed, but poor stability, high cost, and high failure rate due to the local chara

Method used

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  • Rotary scanning real-time fluorescent quantitative PCR (Polymerase Chain Reaction) detection system
  • Rotary scanning real-time fluorescent quantitative PCR (Polymerase Chain Reaction) detection system
  • Rotary scanning real-time fluorescent quantitative PCR (Polymerase Chain Reaction) detection system

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Embodiment Construction

[0030] The present invention is further described below in conjunction with accompanying drawing and example.

[0031] Such as Figure 1-3 As shown, a rotary scanning quantitative fluorescent PCR detection system includes four parts: a fluorescence detection device, a light source excitation device, a thermal cycle module device, and an optical signal transmission device.

[0032] Specifically, the fluorescence detection device includes a stepping motor 1, a motor fixing plate 2, an optical fiber fixing plate 3, an optical path gating rotating plate protection ring 4, an optical path gating rotating plate 5, an optical filter fixing plate 6, and the optical filter is fixed. The disc 6 is provided with a hole for inserting and replacing the optical filter 7 to complete the detection of signals of various wavelengths;

[0033] The output shaft of the stepping motor 1 is connected to the optical path gating rotating plate 5 through the optical fiber fixed disc 3, the optical pat...

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Abstract

The invention provides a rotary scanning real-time fluorescent quantitative PCR (Polymerase Chain Reaction) detection system. The detection system comprises a fluorescent detection device, a light source stimulation device, a thermal cycling module device and a signal transmission device. An output shaft of a stepping motor is connected with a light path gating rotary piece by an optical fiber fixing disc and a light path gating rotary piece protection ring; a base of the fluorescent detection device is provided with a hole corresponding to a detection window of a PMT protection device so that the detection window of the PMT aligns to an optical fiber output end focusing device; coupling barrels are fixed on a coupling barrel fixing device; the coupling barrels are arranged on the coupling barrel fixing device in an array manner of 4*4 uniform arrangement; and the thermal cycling module device comprises a porous reaction tank for inserting a plurality of test tubes, a reaction tank fixing device, a heating refrigeration sheet, a reaction refrigeration heat radiator and a reaction tank refrigeration heat radiation fan which are integrated form top to bottom. According to the rotary scanning real-time fluorescent quantitative PCR detection system, the developed high-power semiconductor heating refrigeration sheet is adopted to realize temperature circulation of a PCR reaction; and the temperature rising speed is high and the amplification efficiency is high.

Description

technical field [0001] The invention belongs to the technical field of molecular biology detection, and specifically relates to DNA segment extension and detection for clinical molecular diagnosis, inspection and quarantine, criminal investigation, agriculture, forestry, animal husbandry, sideline fishery, food safety, and scientific education. Background technique [0002] Polymerase chain reaction (PCR) is a kind of in vitro nucleic acid quantification, that is, DNA fragments are specifically amplified in vitro. A cycle consists of several steps such as high temperature denaturation, low temperature annealing, and suitable temperature extension. The cycle is carried out so that the target DNA can be rapidly amplified. It has the characteristics of strong specificity, high sensitivity, easy operation, and time saving. It is a gene amplification technology. a major innovation. PCR technology can specifically amplify a very small amount of target DNA by millions of times, th...

Claims

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Application Information

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IPC IPC(8): G01N21/64
Inventor 冯继宏白桦娟张弘
Owner BEIJING UNIV OF TECH
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