Rotary scanning real-time fluorescent quantitative PCR (Polymerase Chain Reaction) detection system

Abstract

The invention provides a rotary scanning real-time fluorescent quantitative PCR (Polymerase Chain Reaction) detection system. The detection system comprises a fluorescent detection device, a light source stimulation device, a thermal cycling module device and a signal transmission device. An output shaft of a stepping motor is connected with a light path gating rotary piece by an optical fiber fixing disc and a light path gating rotary piece protection ring; a base of the fluorescent detection device is provided with a hole corresponding to a detection window of a PMT protection device so that the detection window of the PMT aligns to an optical fiber output end focusing device; coupling barrels are fixed on a coupling barrel fixing device; the coupling barrels are arranged on the coupling barrel fixing device in an array manner of 4*4 uniform arrangement; and the thermal cycling module device comprises a porous reaction tank for inserting a plurality of test tubes, a reaction tank fixing device, a heating refrigeration sheet, a reaction refrigeration heat radiator and a reaction tank refrigeration heat radiation fan which are integrated form top to bottom. According to the rotary scanning real-time fluorescent quantitative PCR detection system, the developed high-power semiconductor heating refrigeration sheet is adopted to realize temperature circulation of a PCR reaction; and the temperature rising speed is high and the amplification efficiency is high.

Description

technical field [0001] The invention belongs to the technical field of molecular biology detection, and specifically relates to DNA segment extension and detection for clinical molecular diagnosis, inspection and quarantine, criminal investigation, agriculture, forestry, animal husbandry, sideline fishery, food safety, and scientific education. Background technique [0002] Polymerase chain reaction (PCR) is a kind of in vitro nucleic acid quantification, that is, DNA fragments are specifically amplified in vitro. A cycle consists of several steps such as high temperature denaturation, low temperature annealing, and suitable temperature extension. The cycle is carried out so that the target DNA can be rapidly amplified. It has the characteristics of strong specificity, high sensitivity, easy operation, and time saving. It is a gene amplification technology. a major innovation. PCR technology can specifically amplify a very small amount of target DNA by millions of times, th...

AI Technical Summary

Problems solved by technology

Two-dimensional motor-driven scanning, slow speed, complex electromechanical structure; cooling CCD, fast speed, but poor stability, high cost, and high failure rate due to the local characteristics of the device

The use of two-dimensional area array detectors generally does not require scanning, and the detection speed is fast, but the performance is relatively insufficient

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