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Microporous plate for high throughput detection and application thereof

A microplate, high-throughput technology, used in measurement devices, microbial determination/inspection, color/spectral property measurement, etc., can solve problems such as interference, interference detection specificity, accuracy and sensitivity, and achieve accurate results. Reliable and highly sensitive results

Active Publication Date: 2015-04-29
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Unable to detect high-throughput H production catalyzed by CBS or CSE 2 The reason for S gas is that if it will be used to detect H 2 Adding the Ellman reagent [5,5'-dithiobis(2-nitrobenzoic acid; DTNB) of S gas into the enzyme catalytic system will interfere with the enzyme reaction, and other components in the reaction system will interfere with the specificity and accuracy of the detection sex and sensitivity

Method used

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  • Microporous plate for high throughput detection and application thereof
  • Microporous plate for high throughput detection and application thereof
  • Microporous plate for high throughput detection and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0056] Such as figure 1 and figure 2 As shown, this embodiment provides a microwell plate for high-throughput detection, which is a 96-well plate arranged in a rectangular array of 8×12, including a substrate 1 with 48 microwell groups; each microwell group includes The first micropore 11 and the second micropore 12 are provided with a gas diffusion channel 111 between the first micropore and the second micropore. The micropore is an open-ended hole, which is opened on one surface of the substrate 1;

[0057] The gas diffusion channel 111 is disposed on the micropore wall 110 between the first micropore 11 and the second micropore 12 .

[0058] The first micropore is a reaction hole, and the second micropore is a detection hole. In actual use, the gas diffusion channel allows at least one gas reaction product in the first micropore to enter the second micropore through the gas diffusion channel. hole.

[0059] The gas diffusion channel can be arranged as a circular, squar...

Embodiment 2

[0062] The structure of the microporous plate in this embodiment is basically the same as that in Embodiment 1, except that the gas diffusion channel 111 is provided with a membrane 1111 with selective permeability.

Embodiment 3

[0064] In order to be suitable for high-throughput detection of gaseous target substances produced in biochemical reactions, the microwell plate provided in this example is a 96-well plate arranged in an 8×12 rectangular array, including 48 microwell groups. This example provides a A simple way to set up gas diffusion channels, such as Figure 4 and Figure 5 As shown, the gas diffusion channel is a groove 1112 provided on the top of the micropore wall. In this embodiment, the depth of the groove is half of the depth of the first microhole 11 . In other embodiments, the depth of the groove can be set according to specific conditions, such as selecting an appropriate depth of the groove according to the volume of the liquid in the biochemical reaction system and the detection system, so as to ensure that the liquids in the two systems will not mix through the groove, It can also meet the requirement that the gas generated by the reaction in the first micropore can diffuse int...

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Abstract

The invention discloses a microporous plate for high throughput detection; the microporous plate includes a plurality of micropore groups, each micropore group at least includes a first micropore and a second micropore and also includes a gas diffusion channel communicated with the first micropore and the second micropore. The invention also discloses an application of the microporous plate in high throughput detection of gas generated in a biochemical reaction. The invention provides the microporous plate suitable for high-throughput detection and mutual un-interference detection of biological and / or chemical reactions and target substances, and especially provides the microporous plate for high-throughput screening of an enzyme activity regulator.

Description

technical field [0001] The invention relates to a high-throughput detection device, in particular to a micropore plate suitable for high-throughput detection. Background technique [0002] Microplates, also commonly referred to as multiwell sample trays, are used to hold multiple (eg, 6, 12, 24, 48, 96, 384 wells or more) samples. These samples can be characterized by a variety of techniques such as autoradiography, liquid scintillation counting, luminometry, etc. [0003] Porous microplates are mainly used in the detection of chemical, biological, and pharmaceutical fields to analyze and / or research liquid samples. A standard microplate set typically includes a microplate with a plurality of open wells and an optional closure for sealing the wells. Typically microwell plates generally comprise a single molded structure comprising a rigid frame for housing a plurality of open wells arranged in a rectangular array. Microplates come in a variety of sizes; wells can be as la...

Claims

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Application Information

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IPC IPC(8): G01N33/543G01N21/31
CPCC12Q1/527G01N33/54306
Inventor 吴方周越洋于晶
Owner SHANGHAI JIAO TONG UNIV
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