Applications of cresol in preparation of medicament for inhibiting tumor cell migration and diffusion

A tumor cell and cresol technology, applied in anti-tumor drugs, drug combinations, active ingredients of hydroxyl compounds, etc., can solve the problem of not finding the pharmacological effects of cresol, and achieve the effect of reducing the number of deaths and shortening the research and development cycle.

Inactive Publication Date: 2015-05-06
南京格耀生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Other pharmacological effects of cresol have not been found in the existing open literature

Method used

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  • Applications of cresol in preparation of medicament for inhibiting tumor cell migration and diffusion
  • Applications of cresol in preparation of medicament for inhibiting tumor cell migration and diffusion
  • Applications of cresol in preparation of medicament for inhibiting tumor cell migration and diffusion

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1: The application of cresol in the preparation of drugs for inhibiting the metastasis and spread of colon cancer cells. The specific experimental plan is as follows:

[0025] 1. Experimental materials

[0026] Colon cancer cells (HT29) were used in the experimental stage of the present invention, and the drug used in the positive control was nocodazole (experimental concentration: 10uM). The negative control group received no treatment.

[0027] The experiment used cresol compound C 7 h 8 O, CAS NO. is 1319-77-3, the concentrations are 80uM, 60uM, 40uM, 10uM.

[0028] 2. Experimental method

[0029] For colon cancer cells cultured for 24 hours (cell density: 10 5 ) for drug stimulation for 2 hours:

[0030] (1) Centrifuge the colon cancer cells in a better state in the culture dish, and add an appropriate amount of culture medium to make a cell suspension;

[0031] (2) Get the cell suspension count, according to 10 5 The density was added to a 24-well ...

Embodiment 2

[0039] Example 2: Transwell cell migration assay was used to determine the effect of cresol on the migration ability of colon cancer cells.

[0040] Will 1×10 5 The HT29 cells were inoculated in Transwell chambers with a 12.0 μm membrane at the bottom, and continued to be cultured. After the cells adhered to the wall, DMEM complete medium with different concentrations of cresol was added to the upper chamber, and 0.05%-0.2% BSA was added to maintain the osmotic pressure. The lower chamber was added with DMEM complete medium containing 15% FBS and continued to culture for 24 hours. The unmigrated cells on the upper surface of the membrane were carefully wiped off with a cotton swab, and the cells that migrated to the lower surface of the membrane were fixed with 2% PFA at 37°C for 10 minutes and then stained with Giemsa. Ten visual fields were randomly selected under a high-power microscope to count the migrated cells.

[0041] Cell migration rate = [the average number of cel...

Embodiment 3

[0045] Example 3: The damage repair experiment detects the effect of cresol on the migration ability of tumor cells.

[0046] Take HT29 cells in the logarithmic growth phase, and add 1×10 cells to each well of a 24-well plate 5 After the cells grow into a monolayer, draw a "one"-shaped scratch, wash with PBS, add different concentrations of cresol in the control group and the experimental group, and take pictures. After 22 hours of culture, the same part is taken again to measure the migration distance. The experiment was repeated 3 times, and the cell migration rate=[(0h average value of the experimental group-22h average value of the experimental group) / (0h average value of the blank control group-22h average value of the blank control group)]×100%.

[0047] Cresol inhibited the migration of LTEP and A549 cells at different concentrations (10-80 μg / ml) for 22 hours. The results showed that cresol had a dose-dependent inhibitory effect on colon cancer cell HT29 migration. Co...

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Abstract

The invention discloses applications of cresol in preparation of a medicament for inhibiting tumor cell migration and diffusion. The medicament prepared by utilizing cresol can inhibit the migration capability of the tumor cells, the cresol is a low-toxicity medicament, and can play a large role in blocking the diffusion process of the tumors, and the death rate of normal cells can be greatly reduced by adopting the therapy of other related tumor medicaments; and the majority of the existing tumor medicaments are cytotoxic drugs, and a great amount of normal cells are killed while the tumor cells are killed.

Description

technical field [0001] The invention relates to a new use of cresol in the field of pharmacy, in particular to the use of cresol in the preparation of drugs for inhibiting tumor cell metastasis and spread. Background technique [0002] Cresol is a disinfectant and antiseptic, its antibacterial effect is 3 to 10 times stronger than that of phenol, and its toxicity is almost equal, so its therapeutic index is higher. Cresol can kill bacterial propagules including mycobacteria. 2% solution can kill most pathogenic bacteria after 10-15 minutes, and 2.5% solution can kill Mycobacterium tuberculosis after 30 minutes. Due to its low solubility in water, it is often made into a cresol soap solution (Lysol, Lysol). Cresol soap solution is easy to mix with water and easy to use. [0003] The indications of cresol are: 1. 1%-2% aqueous solution is used for disinfection of hands and skin; 2. 3%-5% solution is used for disinfection of instruments and utensils; 3. 5%-10% solution is us...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/05A61P35/04
Inventor 董梅
Owner 南京格耀生物科技有限公司
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