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Method for preparing high-porosity three-dimensional calcium alginate gel scaffolds by virtue of pulsed ultrasonic wave

A technology of three-dimensional calcium alginate and pulsed ultrasound, which is applied in the direction of tumor/cancer cells, vertebrate cells, animal cells, etc., can solve the problems of organic solvent residue and achieve the effect of simple preparation method

Inactive Publication Date: 2015-05-06
NANJING UNIV +1
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Problems solved by technology

[0006] In order to better simulate the three-dimensional culture environment, the present invention provides a method for preparing three-dimensional calcium alginate with large porosity by using pulsed ultrasound to solve the problems of organic solvent residue and particle filtration in the traditional technique of enhancing the permeability of tissue engineering scaffold materials. The method of the gel support; the present invention uses the cross-linking synthesis method to directly shape the three-dimensional calcium alginate gel support, and irradiates the three-dimensional gel support with a certain intensity of pulsed ultrasound to enhance the micropore connectivity and permeability of the support material , the experiment proves that the three-dimensional gel scaffold with good permeability prepared by the present invention has a good promoting effect on cell growth

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  • Method for preparing high-porosity three-dimensional calcium alginate gel scaffolds by virtue of pulsed ultrasonic wave
  • Method for preparing high-porosity three-dimensional calcium alginate gel scaffolds by virtue of pulsed ultrasonic wave
  • Method for preparing high-porosity three-dimensional calcium alginate gel scaffolds by virtue of pulsed ultrasonic wave

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Embodiment 1

[0032] In conjunction with the accompanying drawings, a method of using pulsed ultrasound to prepare a three-dimensional calcium alginate gel scaffold with large porosity in this embodiment, the specific steps are as follows:

[0033] Step 1. Using the cross-linking synthesis method to directly shape calcium alginate three-dimensional gel scaffolds with different mechanical strengths:

[0034] 1.132g calcium chloride (CaCl 2 ) pure product was dissolved in 100ml deionized pure water, stirred evenly to obtain a 102mmol / L calcium chloride solution, and stored at 4°C. The pure sodium alginate was dissolved in deionized pure water to obtain a 1.2% w / v solution, which was stored at 4°C. The calcium chloride solution with volumes of 100 μl, 200 μl, 300 μl, 400 μl and 500 μl and 500 μl of 1.2% sodium alginate solution were stirred by a vortex mixer, and mixed quickly and thoroughly at a speed of 1800 r / min for 20 seconds in a 15 ml centrifuge tube. The well-mixed solution was place...

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Abstract

The invention discloses a method for preparing high-porosity three-dimensional calcium alginate gel scaffolds by virtue of a pulsed ultrasonic wave, and belongs to the technical field of performance optimization for a three-dimensional scaffold material. The method disclosed by the invention comprises the following steps: directly moulding three-dimensional calcium alginate gel scaffolds with different mechanical strengths by a cross-linking synthesis method; carrying out an applied voltage test on the Young moduli of the three-dimensional gel scaffolds prepared in the step 1 at room temperature, and primarily selecting the three-dimensional gel scaffolds with stable mechanical properties; irradiating the obtained scaffolds by a low-intensity pulsed ultrasonic wave to prepare the high-porosity three-dimensional gel scaffolds, wherein the sound pressure of the low-intensity pulsed ultrasonic wave is 0.055MPa, and the irradiation time is 20min. The three-dimensional gel scaffolds prepared by the method disclosed by the invention are high in porosity and good in permeability, and has a great promotion role on cell growth.

Description

technical field [0001] The invention relates to the technical field of biomedical engineering and performance optimization of three-dimensional scaffold materials, and more specifically relates to a method for preparing a large-porosity three-dimensional calcium alginate gel scaffold by using pulsed ultrasound. Background technique [0002] In order to better simulate the three-dimensional culture environment of cells, the research on three-dimensional scaffold materials has received extensive attention. The traditional two-dimensional cell culture system is different from the in vivo situation, and the cells will gradually lose their original traits. At present, the scaffolds for cell culture are divided into three types: microporous scaffolds, nanofiber scaffolds and hydrogel scaffolds. The microporous scaffold is convenient to use, but its pore size (about 100 μm) is much larger than the average cell diameter (about 10-20 μm), which is similar to the two-dimensional cult...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/09
Inventor 郭各朴屠娟卢璐章东郭霞生邱媛媛
Owner NANJING UNIV
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