Method for preparing high-porosity three-dimensional calcium alginate gel scaffolds by virtue of pulsed ultrasonic wave
A technology of three-dimensional calcium alginate and pulsed ultrasound, which is applied in the direction of tumor/cancer cells, vertebrate cells, animal cells, etc., can solve the problems of organic solvent residue and achieve the effect of simple preparation method
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[0032] In conjunction with the accompanying drawings, a method of using pulsed ultrasound to prepare a three-dimensional calcium alginate gel scaffold with large porosity in this embodiment, the specific steps are as follows:
[0033] Step 1. Using the cross-linking synthesis method to directly shape calcium alginate three-dimensional gel scaffolds with different mechanical strengths:
[0034] 1.132g calcium chloride (CaCl 2 ) pure product was dissolved in 100ml deionized pure water, stirred evenly to obtain a 102mmol / L calcium chloride solution, and stored at 4°C. The pure sodium alginate was dissolved in deionized pure water to obtain a 1.2% w / v solution, which was stored at 4°C. The calcium chloride solution with volumes of 100 μl, 200 μl, 300 μl, 400 μl and 500 μl and 500 μl of 1.2% sodium alginate solution were stirred by a vortex mixer, and mixed quickly and thoroughly at a speed of 1800 r / min for 20 seconds in a 15 ml centrifuge tube. The well-mixed solution was place...
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