Eimeria tenella gametophyte antigen gam59 gene and application thereof

Abstract

The invention belongs to the field of gene engineering vaccines, and concretely relates to an eimeria tenella gametophyte antigen gene, expression and application of an expression product. The size of the open reading frame of the gene is 1617 bp, and the nucleotide sequence of the gene is shown as SEQ ID NO. 1. A prokaryotic expression vector pET28a-Etgam59 is constructed based on the gene, and is transformed into BL21 host bacterium for inducible expression. The expression product can be recognized by chicken convalescent serum, which shows that recombinant protein possesses immunogenicity. The obtained serum of an immunized mouse of a recombinant eukaryotic expression plasmid pcDNA3.1-Etgam59 constructed by amplifying the gene is capable of specifically combined with the expression product of pET28a-Etgam59, which shows that the gene is applicable to development of a chicken coccidiosis gene engineering vaccine.

Description

technical field

[0001] The invention relates to a gametocyte antigen gene of Eimeria tenella, a polypeptide encoded by the gene, a carrier containing the gene, an acquisition method of the gene, an in vitro expression method of the polypeptide and functional identification. Specifically, the gene of the present invention comes from the gametophyte of the sexual reproduction stage of Eimeria tenella. Background technique

[0002] Chicken coccidiosis is a protozoan disease that seriously endangers the chicken industry caused by one or several coccidia of the genus Eimeria parasitizing in the small intestine or cecum mucosa of chickens. It is estimated that the annual global loss due to coccidiosis is 3 billion Dollar. The annual consumption of anticoccidiostats alone in my country is about 600-1.8 billion yuan, and the direct and indirect economic losses caused by chicken coccidiosis are difficult to estimate. For a long time, the prevention and treatment of chicken coccidio...

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