Salmonella paratyphi ompN gene prokaryotic expression system and application of recombination expression protein thereof

A technology for paratyphoid and salmonella, applied in the application, genetic engineering, plant genetic improvement and other directions, can solve the problems of difficult to obtain immune protection effect, poor immune effect and other problems, achieve easy cultivation and fermentation, simple structure, fast growth rate Effect

Inactive Publication Date: 2015-05-06
ZHEJIANG MEDICAL COLLEGE +1
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

Bacteria are prokaryotic microorganisms with complex and diverse surface antigens. It is often difficult to obtain a satisfactory immune protection effect by using a single protein antigen. This may be the main re

Method used

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  • Salmonella paratyphi ompN gene prokaryotic expression system and application of recombination expression protein thereof
  • Salmonella paratyphi ompN gene prokaryotic expression system and application of recombination expression protein thereof
  • Salmonella paratyphi ompN gene prokaryotic expression system and application of recombination expression protein thereof

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Embodiment Construction

[0036] The present invention will be further described below in conjunction with the examples.

[0037] 1 Materials and methods

[0038] 1.1 Sources of strains and serum samples

[0039]Salmonella paratyphi A reference standard strain 50001 was purchased from Beijing China Institute for the Control of Pharmaceutical and Biological Products. 126 clinical strains of Salmonella paratyphi A, 56 convalescent sera from patients with paratyphi A were provided by the Centers for Disease Control and Prevention in Ningbo, Wenling and Hangzhou, Zhejiang Province, and 15 healthy subjects with negative microveda test results Serum was provided by Zhejiang Xinhua Hospital.

[0040] 1.2 ompN Gene amplification and sequencing

[0041] The bacterial genomic DNA extraction kit (Axygen) was used to extract the above-mentioned Salmonella paratyphi A genomic DNA, and its concentration was determined by ultraviolet spectrophotometry [13] . According to Salmonella paratyphi A ATCC9150 strain i...

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Abstract

The invention discloses a salmonella paratyphi ompN gene prokaryotic expression system and application of recombination expression protein thereof, and belongs to the technical field of biologics. The nucleotide sequence of the salmonella paratyphi ompN gene is shown in the SEQ ID No: 1. The amino acid sequence of the recombination expression protein of the salmonella paratyphi ompN gene is shown in the SEQ ID NO: 2. The salmonella paratyphi ompN gene disclosed by the invention is wide in distribution and conservative in sequence, the recombination expression product rOmpN has strong immunogenicity and immunoprotection, and can be used as multivalent salmonella paratyphi genetic engineering vaccine candidate antigen.

Description

technical field [0001] The invention belongs to the field of biological technology, and in particular relates to the prokaryotic expression system of Salmonella paratyphi A ompN gene and the application of the recombinant expression protein. Background technique [0002] Typhoid fever and paratyphoid fever are caused by Salmonella typhi, Salmonella paratyphi A, Salmonella scottmueller, and occasionally Salmonella hirschfeldii Common human acute infectious diseases. Every year, the number of cases of typhoid or paratyphoid fever in the world is as high as about 20 million, and the number of deaths exceeds 200,000. Typhoid fever or paratyphoid fever is also one of the common statutory B infectious diseases in my country. The annual incidence rate is among the top 5 of all B infectious diseases. Therefore, it is one of the infectious diseases that my country focuses on monitoring and immunization. [0003] Vaccination is the fundamental measure for the prevention and control ...

Claims

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Application Information

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IPC IPC(8): C12N15/31C07K14/255A61K39/112A61P31/04C12N15/70C12N15/66C12N1/21C12R1/42
Inventor 孙爱华严杰
Owner ZHEJIANG MEDICAL COLLEGE
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