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Sansevieria trifasciata in-vitro culture one-step seedling formation culture medium and culture method

A technology of in vitro culture and medium formula, which is applied in the field of one-step seedling growth medium and culture of Phnom Penh lichen in vitro culture, can solve the problems of low reproduction coefficient, long culture period, difficult to obtain a large number of plants, etc.

Active Publication Date: 2015-05-13
RIZHAO POLYTECHNIC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The common propagation methods of Sansevieria Phnom Penh include leaf cutting method, branching method, etc. These conventional propagation methods have low reproduction coefficient, long cultivation period, and it is not easy to obtain a large number of plants, and there will be a phenomenon of species degradation. The traits of Sansevieria Phnom Penh Easy to disappear, affecting viewing quality

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  • Sansevieria trifasciata in-vitro culture one-step seedling formation culture medium and culture method

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specific Embodiment approach 1

[0013] Specific implementation mode 1: This implementation mode will further describe and illustrate the present invention in conjunction with specific examples below.

[0014] A kind of Sansevieria Phnom Penh in vitro culture one-step seedling medium, using MS as the basic medium, adding different concentrations of 2,4-dichlorophenoxyacetic acid, naphthaleneacetic acid, kinetin combination, wherein 2,4-dichlorobenzene The concentration of oxyacetic acid is 0.2mg / L, 0.4mg / L or 0.6mg / L, the concentration of naphthaleneacetic acid is 0.0mg / L, 0.1mg / L or 0.2mg / L, the concentration of KT is 0.5mg / L, 1.0 mg / L or 1.5mg / L.

[0015] The optimal formulation of the medium was 0.2mg / L of 2,4-dichlorophenoxyacetic acid, 0.1mg / L of naphthaleneacetic acid and 1.0mg / L of KT.

[0016] Or the optimum formula of the medium is 0.2 mg / L of 2,4-dichlorophenoxyacetic acid, 0.2 mg / L of naphthalene acetic acid and 1.5 mg / L of KT.

[0017] After 40-50 days of culture medium, it can directly develop ...

specific Embodiment approach 2

[0019] Specific embodiment two: culture method is as follows, step 1, prepare Sansevieria one-step seedling medium, and carry out sterilization process;

[0020] Step 2: Take the young leaves of Hupilan, which are free from diseases and insect pests, wash them with running water for 1 hour, soak them in 75% alcohol for 15 seconds, and use 0.1% HgCl 2 After being treated with disinfectant for 5 minutes, rinsed with sterile water several times, cut into 1cm×1cm blocks with a scalpel;

[0021] Step 3. Inoculate the explants sterilized in Step 2 into the seedling culture medium, and place them at a temperature of 25±2°C for cultivation, with a light time of 12-14 hours per day and a light intensity of 2000-3000lx;

[0022] Step 4, after 40-50 days of light, regenerated plants of tiger Pilan are obtained.

[0023] Adopt following test to verify the effect of this embodiment:

[0024] With MS as the basic medium, three factors and three levels of L 9 (3 4 ) of orthogonal experim...

Embodiment 2

[0037] Utilize described Sansevieria one-step seedling-forming medium, the culture method that makes Sansevieria leaf in vitro culture one-step seedling-forming, comprises the following steps:

[0038] 1) Prepare Sansevieria one-step seedling growth medium, add 0.2mg of 2,4-dichlorophenoxyacetic acid, 0.1mg of naphthaleneacetic acid, and 1.0mg of kinetin to each liter of medium MS medium, perform sterilization treatment, and sterilize The temperature is 121°C and the sterilization time is 15 minutes.

[0039] 2) Select Sansevieria leaves that grow vigorously and are free from diseases and insect pests, rinse with running water for 1 hour, soak in 75% alcohol for 15 seconds, rinse with sterile water several times, and use 0.1% HgCl 2 After being treated with disinfectant for 5 minutes, rinsed with sterile water several times, cut into 1cm×1cm blocks with a scalpel.

[0040] 3) Inoculate the leaf explants that have been sterilized in step (2) into the best medium for one-step s...

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Abstract

The invention discloses a sansevieria trifasciata in-vitro culture one-step seedling formation culture medium and a culture method, and belongs to the technical field of plant tissue culture, for solving the problems that sansevieria trifasciata bred by a conventional method is low in coefficient, and slow in growth and cannot form large-scale production. The method comprises the following steps: step 1, disinfecting and sterilizing a culture medium; step 2, disinfecting and sterilizing sansevieria trifasciata blades and seedlings; step 3, inoculating disinfected and sterilized explants into a seedling culture medium, and culturing at the temperature condition of 25+ / -2 DEG C, wherein the illumination time each day is 12-14 hours, and the illumination intensity is 2000-3000lx; and step 4, illuminating for 40-50 days to obtain a sansevieria trifasciata regenerated plant. The sansevieria trifasciata in-vitro culture one-step seedling formation culture medium and the culture method are used for sansevieria trifasciata in-vitro tissue culture.

Description

technical field [0001] The invention relates to a one-step seedling growth medium and a cultivation method for in vitro culture of Sansevieria Phnom Penh, belonging to the technical field of plant tissue culture. Background technique [0002] Sansevieria trifasciata var.harnii, also known as Sansevieria trifasciata var. harnii, is a perennial evergreen succulent herb belonging to the genus Sansevieria trifasciata in the Agaveaceae family. Native to tropical Africa and India, it is a herbaceous foliage plant that can purify the indoor environment. Its leaf color is beautiful, and the arrow-shaped leaves are tall and straight, with strong adaptability. [0003] The common propagation methods of Sansevieria Phnom Penh include leaf cutting method, branching method, etc. These conventional propagation methods have low reproduction coefficient, long cultivation period, and it is not easy to obtain a large number of plants, and there will be a phenomenon of species degradation. Th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 李媛侯可雷
Owner RIZHAO POLYTECHNIC
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