Method for preparing bacterial cellulose by using bean curd yellow serofluid

A technology for bacterial cellulose and yellow pulp water, which is applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as environmental pollution of tofu yellow pulp water, and achieve the effects of simple and convenient pretreatment and increased yield.

Inactive Publication Date: 2015-05-13
哈尔滨瑞康源生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to overcome the deficiencies of the above-mentioned prior art, solve the problem of environmental pollution of tofu yellow pulp water...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment （1）

[0010] Example (1) Optimum preparation of cellulose medium: choose tofu yellow pulp water as the stock solution, first use 5000r / min centrifugation for 10 minutes to remove impurities in the tofu yellow pulp water, and directly take the supernatant liquid for later use.

[0011] (2) Preparation of medium: slant solid medium: 30g glucose, 10g yeast extract powder, 15g agar, 1L distilled water, pH5.5, sterilized at 121°C for 15min; liquid seed medium: 30g glucose, 10g yeast extract powder, Distilled water 1L, pH 5.5, sterilized at 121°C for 15 minutes; fermentation medium: tofu yellow pulp water medium, pH 5.5, sterilized at 121°C for 15 minutes.

[0012] (3) Preparation of bacterial cellulose: Bacterial cellulose was produced with tofu yellow pulp water as the fermentation substrate, 8% glucose was added, the fermentation time was 7 days, the fermentation temperature was 30°C, the inoculation amount was 6%, and the initial pH was 5.0. Under these conditions, the yield of bac...

Embodiment 2

[0014] (1) Optimal preparation of cellulose medium: choose tofu yellow pulp water as the stock solution, firstly use 5000r / min centrifugation for 10min to remove the impurities in the tofu yellow pulp water, and directly take the supernatant liquid for later use.

[0015] (2) Preparation of medium: slant solid medium: 30g glucose, 10g yeast extract powder, 15g agar, 1L distilled water, pH5.5, sterilized at 121°C for 15min; liquid seed medium: 30g glucose, 10g yeast extract powder, Distilled water 1L, pH 5.5, sterilized at 121°C for 15 minutes; fermentation medium: tofu yellow pulp water medium, pH 5.5, sterilized at 121°C for 15 minutes.

[0016] (3) Preparation of bacterial cellulose: Bacterial cellulose was produced with tofu yellow pulp water as the fermentation substrate, 8% glucose was added, the fermentation time was 7 days, the fermentation temperature was 30°C, the inoculation amount was 6%, and the initial pH was 5.0. Under these conditions, the yield of bacterial ...

Embodiment 3

[0018] (1) Optimal preparation of cellulose medium: choose tofu yellow pulp water as the stock solution, firstly use 5000r / min centrifugation for 10min to remove the impurities in the tofu yellow pulp water, and directly take the supernatant liquid for later use.

[0019] (2) Preparation of medium: slant solid medium: honey 30g, yeast extract powder 10g, agar 15g, distilled water 1L, pH5.5, sterilized at 121°C for 15min; liquid seed medium: honey 30g, yeast extract powder 10g, Distilled water 1L, pH 5.5, sterilized at 121°C for 15 minutes; fermentation medium: tofu yellow pulp water medium, pH 5.5, sterilized at 121°C for 15 minutes.

[0020] (3) Preparation of bacterial cellulose: Bacterial cellulose was produced with tofu yellow pulp water as the fermentation substrate, 9% honey was added, the fermentation time was 7 days, the fermentation temperature was 30°C, the inoculation amount was 6%, and the initial pH was 5.0. Under these conditions, the yield of bacterial cellul...

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PUM

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Abstract

A method for preparing bacterial cellulose by using bean curd yellow serofluid is characterized in that bean curd yellow serofluid is adopted as a stock solution, and a centrifuge is adopted to remove impurities from the bean curd yellow serofluid and purify a culture solution; a slant solid medium is prepared by using 40g of glucose, 10g of yeast extract powder, 15g of agar and 1L of distilled water, the pH value of the slant solid medium is 4.5, and the slant solid medium is disinfected at 121DEG C for 15min; a liquid seed medium is prepared by using 40g of glucose, 10g of the yeast extract powder and 1L of distilled water, the pH value of the liquid seed medium is 4.5, and the liquid seed medium is disinfected at 121DEG C for 15min; a bean curd yellow serofluid medium is disinfected at 121DEG C for 15min; and 8% of glucose is added, fermentation is carried out at 30DEG C for 7d, the inoculation amount is 6%, and the initial pH value is 5.0. The bacterial cellulose output is 1.40g/100mL under above conditions, and the glucose utilization rate is 97.5%. The method has the advantages of simple process, low cost, extremely high yield, environmental pollution reduction, and good social and economic benefits, and can be widely used for preparing bacterial cellulose by using bean curd yellow seroflui.

Description

technical field [0001] The invention relates to a method for preparing bacterial cellulose, in particular to a method for preparing bacterial cellulose with tofu yellow slurry water. Background technique [0002] Tofu yellow pulp water is the wastewater discharged from soybean product factories. Due to the limitation of process conditions and processing equipment, a large amount of yellow pulp water is discarded, which not only causes waste of resources but also pollutes the environment. In recent years, my country's soybean product industry has developed rapidly. According to the preliminary statistics of the National Soybean Products Professional Committee, in 2008 alone, the total input of soybeans was 284,400 tons, and every ton of soybeans processed will discharge 2 to 5 tons of yellow pulp water. Therefore, , a large amount of yellow slurry water will be discarded every year. The protein content in yellow pulp water is about 0.3%, the fat content is about 0.08%, and th...

Claims

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Application Information

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IPC IPC(8): C12P19/04C12R1/02
Inventor 夏秀芳黄莉王英男吴磊
Owner 哈尔滨瑞康源生物科技有限公司
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