Method for forming seedlings by carrying out tissue culture on echinochloacrus-galli

Abstract

The invention relates to a method for forming seedlings by carrying out tissue culture on Echinochloacrus-galli. The method comprises the steps of by taking mature echinochloacrus-galli seeds as explant, removing part of endosperm, carrying out treatments such as water absorption, disinfection and the like, and putting the seeds on a callus inducing medium under the dark condition at the temperature of 28 DEG C, wherein the callus grows after 7 days; then, putting the callus on a subculture medium, and culturing for 7 days; transferring the subcultured callus into a differential medium, and culturing for 14 days; under the same temperature and light conditions, transferring the differentiated seedlings into a rooting medium, and culturing for 7 days; watering the seedlings by tap water in the air for 3 days, and transplanting the seedlings into soil to obtain the tissue culture seedlings of the echinochloacrus-galli. According to the method, the mature echinochloacrus-galli seeds are taken as the explant, the echinochloacrus-galli seeds are easily obtained, echinochloacrus-galli is high in seed yield, easy to store and easy in induce regeneration, and the processes of dedifferentiation and redifferentiation are easy to master, so that the method is good in experimental repeatability and high in rooting rate.

Description

(1) Technical field [0001] The invention relates to a method for culturing barnyardgrass tissue to grow seedlings. (2) Background technology [0002] Barnyardgrass (Echinochloacrus-galli) is a worldwide malignant weed in farmland, which is mainly controlled by chemical pesticides. Due to the continuous use of a single herbicide for many years, barnyardgrass has developed resistance to 10 types of herbicides with different mechanisms of action (Heap, 2015). Resistant barnyardgrass has caused problems such as increased use of pesticides, residues, and pollution, which severely restrict the sustainable development of agriculture and become a problem in agricultural production. Studying the resistance mechanism of barnyardgrass is an important way to solve the problem of resistance. Among them, barnyardgrass transgene is the main technology to verify the function of drug-resistant genes, but there is currently a lack of methods to obtain high-efficiency barnyardgrass receptor system...

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problems in the prior art of using barnyardgrass inflorescences as explants to obtain embryoid bodies and then develop into seedlings, the test is difficult, takes a long time, has poor repeatability, and is not suitable for large-scale cultivation. In the present invention, mature embryos of barnyardgrass are used as explants Through the study of seed treatment, callus induction, bud differentiation, rooting culture and hormone selection, explore the method of obtaining barnyardgrass regenerated seedlings through tissue culture, establish the plant receptor system in the process of barnyardgrass gene transformation, and provide a basis for barnyardgrass tissue culture. and transgenic research provide basic information

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