Method for regenerating cell and virus culture microcarrier

Abstract

The invention relates to a method for regenerating a cell and virus culture microcarrier. According to the method, the used microcarrier, namely the microcarrier which has been used for culturing adherent cells, is effectively treated through a special treatment solution and an appropriate treatment means, to achieve the same culture effect as a new microcarrier. Moreover, the microcarrier can be reused for 4 to 5 times through the treatment of the method, and the cell and virus culture effect of the microcarrier used for the 5th time still achieves more than 90% of the culture effect of the new microcarrier. Thus, by establishing the method, the utilization rate of the microcarrier is improved by nearly 4 times, and the cost for utilizing the microcarrier in a large scale to culture cells and viruses is greatly reduced. The microcarrier regenerating method can be widely used for treating other microcarriers used in cell culture.

Description

technical field [0001] The invention relates to a method for regeneration treatment of microcarriers for cell and virus culture, which belongs to the field of biotechnology. Background technique [0002] Since the 1960s, with the continuous enrichment and improvement of cell biology, culture systems and culture methods, animal cell in vitro culture technology has developed by leaps and bounds, and has now become a widely used technology in biomedicine and medical research. method. At present, many vaccines, monoclonal antibodies, cytokines, protein drugs and enzymes with important medical value have been produced by using animal cell culture technology. [0003] Undoubtedly, large-scale animal cell culture technology has become a very important link in biotechnology pharmaceuticals. Microcarriers are the most adherent animal cell adhesion media in the field of cells, and their quality will directly affect the growth of cells, and even affect the proliferation of viruses th...

AI Technical Summary

Problems solved by technology

However, due to the immaturity of microcarrier manufacturing technology in our country, the source of microcarriers can only rely on foreign imports.

Imported microcarriers are expensive due to the monopoly of technology, so that in the large-scale cell or virus culture process, the cost of microcarriers accounts for most of the R&D and production costs, which has caused great harm to the development of domestic biopharmaceutical technology. influences

[0005] In order to reduce this part of the cost, and to avoid the termination of the culture due to accidental reasons during the large-scale cell or virus culture process, the microcarriers are not fully utilized and discarded

Many companies that use microcarriers to cultivate cells or viruses on a large scale to develop and produce biomedicine have explored and established repeated treatment methods for used microcarriers (such as patent application No. 201210075589.2, and the literature "Research on Microcarrier Regeneration" (Authors: Li Zhiqiang, Wang Yan, Guan Guifan, Li Yunfu, etc., published in "Chinese Journal of Biological Products" No. 7, No. 3, 1994)), but for the use of these methods to deal with the regenerated microcarriers, there is no effect on cell culture. or viral culture effects

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