A method for regenerating microcarriers for cell and virus culture

Abstract

The invention relates to a regeneration treatment method for microcarriers used for cell and virus culture. The method is to effectively treat the used microcarriers, that is, the microcarriers that have cultured adherent cells, through special treatment liquid and appropriate treatment means, so as to achieve the same culture effect as the new microcarriers. And through the treatment of this method, the microcarrier can be used repeatedly 4 to 5 times, even if the treated microcarrier is used for the 5th time, the cell and virus culture effect still reaches more than 90% of the culture effect of the new microcarrier. Therefore, the establishment of this method not only increases the utilization rate of microcarriers by nearly 4 times, but also greatly reduces the cost of cultivating cells and viruses using microcarriers on a large scale. The microcarrier regeneration treatment method described in the present invention can be widely used to treat other microcarriers used in cell culture.

Description

Technical field [0001] The invention relates to a regeneration treatment method of microcarriers for cell and virus culture, which belongs to the field of biotechnology. Background technique [0002] Since the 1960s, with the continuous enrichment and improvement of cell biology, culture systems and culture methods, animal cell in vitro culture technology has been developed by leaps and bounds, and has now become a widely used technology in biomedicine and medical research. method. At present, many vaccines, monoclonal antibodies, cytokines, protein drugs and enzyme substances with important medical value have been produced using animal cell culture technology. [0003] Undoubtedly, large-scale animal cell culture technology has become a very important part of biotechnology pharmaceuticals. As the most adherent animal cell attachment medium in the cell field, microcarriers, their quality will directly affect the growth of cells, and even the proliferation of viruses that use cell...

AI Technical Summary

Problems solved by technology

However, due to the immaturity of microcarrier manufacturing technology in our country, the source of microcarriers can only rely on foreign imports.

Imported microcarriers are expensive due to the monopoly of technology, so that in the large-scale cell or virus culture process, the cost of microcarriers accounts for most of the R&D and production costs, which has caused great harm to the development of domestic biopharmaceutical technology. influences

[0005] In order to reduce this part of the cost, and to avoid the termination of the culture due to accidental reasons during the large-scale cell or virus culture process, the microcarriers are not fully utilized and discarded

Many companies that use microcarriers to cultivate cells or viruses on a large scale to develop and produce biomedicine have explored and established repeated processing methods for used microcarriers (such as patent application No. 201210075589.2, and the literature "Research on Microcarrier Regeneration" (Authors: Li Zhiqiang, Wang Yan, Guan Guifan, Li Yunfu, etc., published in "Chinese Journal of Biological Products", No. 7, No. 3, 1994)), but for the use of these methods to deal with the regenerated microcarriers, there is no effect on cell culture. or viral culture effects

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