COI gene sequence of species of coral reef benthos and species identification method utilizing COI gene sequence

A benthic organism and gene sequence technology, applied in the fields of taxonomy and molecular biology, can solve the problems of lack of COI sequence information and less sequence information

Inactive Publication Date: 2015-05-20
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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Problems solved by technology

However, at present, there is a lack of COI sequence information on the benthic organisms in coral reefs in China's seas in domestic and foreign species gene banks or published gene sequence data. Even for community-dominant species and common species, there is very little sequence information for reference. Therefore, Even if researchers determine the COI sequence of an unknown species, there is no way to compare and obtain identification results

Method used

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  • COI gene sequence of species of coral reef benthos and species identification method utilizing COI gene sequence
  • COI gene sequence of species of coral reef benthos and species identification method utilizing COI gene sequence
  • COI gene sequence of species of coral reef benthos and species identification method utilizing COI gene sequence

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Embodiment 1

[0062] 1. Extraction of DNA template.

[0063] Take about 100mg of the muscle tissue of the sample to be tested (a sample of the dominant species in the community or a species with a large number of individuals), cut it into pieces or grind it evenly, and put it into a 1.5mL centrifuge tube. Use the Marine Animal Tissue Genomic DNA Extraction Kit (or any other effective method for extracting DNA) and follow the steps in the instructions to extract the genomic DNA of the sample. The obtained DNA was dissolved in TE buffer or ultrapure water (the pH of the solution was adjusted to about 8.0 with NaOH), and stored in a -20°C refrigerator. The extracted DNA was checked by 1.5% agarose gel electrophoresis, ethidium bromide (EB) staining and observation under ultraviolet light.

[0064] 2. PCR amplification of the COI gene sequence of the sample to be tested. The PCR reaction system for the COI gene sequence is as follows: a total volume of 50 μL. Among them, 5 μL of 10×PCR buffer ...

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Abstract

The invention belongs to the field of biosystematics and molecular biologics, and in particular discloses a cytochrome C oxidase O gene I (COI) gene sequence of species of coral reef benthos and a species identification method utilizing the COI gene sequence. The standard sequences of the COI gene of different dominant species and common species of Hainan coral reef benthos are as shown in the nucleotide sequences of SEQ ID NO.1-14. On the basis of massive study and investigation, the standard COI gene sequences of 14 dominant species and common species of Hainan coral reef benthos are provided, and meanwhile by adopting the method, the species can be rapidly and effectively identified.

Description

technical field [0001] The invention belongs to the field of biological taxonomy and molecular biology, in particular to a cytochrome C oxidase I gene (COI) sequence of various species of coral reef benthic organisms and a species identification method using the COI gene sequence. Background technique [0002] The coral reef ecosystem is one of the ecosystems with the highest biodiversity in the world, and the complex and diverse biological communities inhabiting it also make it a hot spot for marine biology and ecology research. At the same time, the coral reef ecosystem is also the most fragile and disturbed ecosystem in the ocean. It is currently being disturbed by various pressures, including ocean acidification caused by global warming and human activities, such as the discharge of land-based pollutants. , marine engineering construction, fishing and collection activities, etc., have greatly affected the growth and reproduction of reef-building corals, and directly caus...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/53C12Q1/68
Inventor 董栋寇琦李新正
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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