Method for enhancing antigen response of dendrite cells by modifying dendrite cells

An antigen and cell technology, applied to cells modified by introducing foreign genetic material, other methods of inserting foreign genetic material, recombinant DNA technology, etc., can solve problems such as lack of antigen effect

Inactive Publication Date: 2015-05-20
陶英亮
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These approaches are effective at enhancing MHC class II presentation of endogenous antigens but are less effective at antigens naturally produced by antigen-presenting cells

Method used

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  • Method for enhancing antigen response of dendrite cells by modifying dendrite cells
  • Method for enhancing antigen response of dendrite cells by modifying dendrite cells
  • Method for enhancing antigen response of dendrite cells by modifying dendrite cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] MHC class I presentation of antigen: GFP mRNA was introduced into DC by electroporation, and the expression of GFP was detected by flow cytometry after 24 hours. Electroporation of mRNA is a method for efficiently expressing and presenting antigens in human DCs. like figure 1 As shown in A, GFP mRNA efficiently transfected bone marrow-derived mouse DCs. Voltage and duration of electroporation affect transfection efficiency. like figure 1 B demonstrates the presentation of major MHC class I-restricted OVA peptides in DCs that have been electroporated with OVA mRNA or GFP mRNA. Presentation assays were performed with RF33.70T-hybridoma cells. Transfection of DCs with OVA was performed in the presence of DMRIE-C lipids, or pulsed with MHC class I-restricted OVA or VSV peptides. like figure 1 B shows the process of electroporation of mRNA encoding chicken OVA cDNA into DCs and presentation of major OVA epitopes to corresponding T-hybridoma cells. The efficiency of OV...

Embodiment 2

[0033] Treatment of DCs with antisense oligonucleotides to suppress their invariant strand expression: Two phosphorylation-modified antisense oligonucleotides (ODNs) were used to suppress the expression of li in mouse DCs. On day 7, OVA mRNA and 50 nM li AS ODN (AE40) or control ODN (SE40) were introduced into DCs by electroporation. After 48 hours, the cells were stained with FITC-labeled anti-mouse CD74(li) antibody to detect the expression of li on the cell surface. The result is as figure 2 As shown in A. Black histograms show the results of li AS ODN treatment, and blank histograms represent the results of control ODN treatment. Dashed histograms represent DCs stained with FITC-labeled antibodies of the same type. Cells were permeabilized prior to staining with antibodies to detect total li expression. figure 2 A shows that the expression of li(CD74) on the surface of DCs was significantly reduced after co-incubation with antisense oligonucleotides, but not in the c...

Embodiment 3

[0035] Presentation of MHC class I and class II OVA epitopes: Transfection of DCs with OVA mRNA inhibited li synthesis, thereby enhancing MHC class II presentation of OVA. The DCs used were transfected with OVA mRNA or truncated OVA mRNA (tOVA) in which the first 40 nucleic acids were cleaved. DCs transfected with OVA mRNA were also incubated with AE40 or SE40. The processing and presentation of class I and class II epitopes by OVA mRNA-transfected DCs was determined by the T hybridoma cells specific for the respective epitopes used. C57BL / 6(H-2B) mice present an H-2Kb class I-restricted epitope, as well as an I-Ab-restricted class II epitope. like image 3 As shown in A, the expression of class II antigen epitopes was not detected in the tOVA group. In contrast, for native and secreted OVA, class II epitopes are processed for presentation to class II-restricted T-hybridoma cells. Co-incubation of li antisense primers with OVA mRNA-transfected DCs enhanced the expression o...

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Abstract

The invention discloses a method for enhancing the antigen response of dendrite cells (DC) through inhibiting the DC and the expression of invariant chain protein (li) in modified DC.

Description

technical field [0001] The present invention relates to a method for enhancing the response of dendritic cells (DC) to antigen cells by inhibiting the expression of invariant (li) in dendritic cells (DC). Background technique [0002] Dendritic cells (DCs) are specialized cells that present antigens to immature or dormant T cells, so they play an important role in both T cell and B cell immunity. DC immunization induced by tumor antigens is an effective method to induce anti-tumor immunity. An efficient way to load DCs with tumor antigens is to transduce DCs together with recombinant viral vectors or to transfect them with mRNA encoding tumor antigens. It has been demonstrated that mouse and human DCs transfected with mRNA can stimulate cytotoxic T lymphocyte (CTL) responses both in vitro and in vivo. Treatment of tumor-bearing mice with DCs transfected with tumor RNA resulted in decreased tumor metastasis and improved survival. In a phase I clinical trial, by transfectin...

Claims

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Application Information

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IPC IPC(8): C12N15/87C12N5/10
Inventor 陶英亮
Owner 陶英亮
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