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Blood stabilizer and application thereof

A blood stabilizer and blood technology, applied in the field of blood stabilizers, can solve the problem of not being able to provide DNA/RNA denaturation, etc., and achieve the effects of preventing nucleated cells from breaking, avoiding concentration increase, and preventing degradation

Active Publication Date: 2015-05-20
GUANGDONG ASCENDAS GENOMICS TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although Kryofix has been used successfully, it does not provide adequate protection against DNA / RNA denaturation

Method used

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  • Blood stabilizer and application thereof
  • Blood stabilizer and application thereof
  • Blood stabilizer and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] An embodiment of the blood stabilizer described in the present invention, the blood stabilizer described in the present embodiment comprises the components of the following concentrations:

[0026]

[0027] Wherein, the concentrations of the potassium dichromate and lecithin are mass percent concentrations.

[0028] The preparation method of the blood stabilizer described in the present embodiment is: at first, calculate β-cyclodextrin (molecular weight is 1135), disodium cromoglycate ( Molecular weight is 248.3), triflusal (molecular weight is 248.2), EDTA tripotassium salt (molecular weight is 406.5), and according to the total mass of blood stabilizer and the mass percentage concentration of its components, potassium dichromate and lecithin are calculated Then, weigh each component in the blood stabilizer according to the calculation result; finally, add the weighed components into a volumetric flask, add water to dissolve, and constant volume.

Embodiment 2

[0030] An embodiment of the blood stabilizer described in the present invention, the blood stabilizer described in the present embodiment comprises the components of the following concentrations:

[0031]

[0032]

[0033] Wherein, the concentrations of the potassium dichromate and lecithin are mass percent concentrations.

[0034] The preparation method of the blood stabilizer described in this embodiment is the same as that in Example 1.

Embodiment 3

[0036] An embodiment of the blood stabilizer described in the present invention, the blood stabilizer described in the present embodiment comprises the components of the following concentrations:

[0037]

[0038] Wherein, the concentrations of the potassium dichromate and lecithin are mass percent concentrations.

[0039] The preparation method of the blood stabilizer described in this embodiment is the same as that in Example 1.

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Abstract

The invention relates to a blood stabilizer and application thereof. The blood stabilizer is used for stabilizing a blood sample. The blood stabilizer disclosed by the invention comprises the following components according to concentrations: 10-300micromol / L of beta-cyclodextrin, 15-350micromol / L of disodium cromoglycate, 0.01%-0.5% of potassium dichromate, 5-150micromol / L of triflusal, 0.05%-1.5% of lecithin and 2.5-5.0mumol / L of EDTA tripotassium salt, wherein the concentration of potassium dichromate and lecithin is mass percent concentration. The blood stabilizer is capable of stabilizing hemocyte, preventing karyocyte from cracking to avoid releasing DNA in cells, and avoiding increasing the concentration of DNA outside the hemocyte; and meanwhile, the blood stabilizer disclosed by the invention is capable of preventing in vitro degradation of the DNA and preventing blood coagulation.

Description

technical field [0001] The invention relates to a blood stabilizer and an application thereof, in particular to a blood stabilizer capable of protecting blood cell membranes and resisting DNA degradation and an application thereof. Background technique [0002] Stabilizing blood samples is extremely important for the transportation of blood samples and the operation of the clinical trial process. After adding common anti-blood and anti-coagulant reagents when collecting whole blood samples, the blood needs to be analyzed or processed within 6 hours (no more than 12 hours at most). [0003] Formaldehyde, as a fixative, is the mainstay of clinical and research tissue and biological sample preservation. The rationale for the use of formaldehyde as a fixative is its propensity to form cross-links between structurally organized proteins and protein moieties of lipoproteins and glycoproteins. Formaldehyde, as a fixative, has some disadvantages. First of all, the slow penetratio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28
Inventor 邓杏飞
Owner GUANGDONG ASCENDAS GENOMICS TECH CO LTD
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