Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of bone carbuncle rabbit model and application thereof

A model, the technology of the tibia, applied in the field of experimental animals, achieves the effect of good application prospects, good animal activities and eating conditions, and small surgical trauma

Inactive Publication Date: 2015-05-27
ZHEJIANG ACAD OF TRADITIONAL CHINESE MEDICINE
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to literature search, there are currently no literature reports or patent publications on the method of preparing a rabbit model of Fugu carbuncle by combining physical stimulation with bacterial infection and its application in the evaluation of the treatment effect of Fugu carbuncle nonunion.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of bone carbuncle rabbit model and application thereof
  • Preparation method of bone carbuncle rabbit model and application thereof
  • Preparation method of bone carbuncle rabbit model and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0044] A detailed description will be given below of specific embodiments of the present invention.

[0045] A preparation method and application thereof of a carbuncle model rabbit, the method comprises the following steps:

[0046] 1) Animal selection and feeding conditions

[0047] New Zealand rabbits, male, weighing 2.25±0.25 kg, animal feeding and overall experiments were completed in a barrier-level experimental animal center;

[0048] 2) Recovery and cultivation of strains

[0049] Take the standard strain of Staphylococcus aureus (ATCC 25923 Staphylococcus aureus subsp. aureus ), add 0.3mL nutrient gravy agar medium, shake gently to dissolve the freeze-dried bacteria and form a suspension, absorb all the bacterial suspension, transplant it into a nutrient gravy agar medium test tube, and store it at 37℃ Under oxygen condition, cultivate for 24h. Take the test tube and place it in XXX, and perform turbidimetry with a standard turbidimetric tube with a concen...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a preparation method of a bone carbuncle rabbit model and an application of the model to evaluating bone nonunion treating effects of bone carbuncles, which belong to the field of experimental animals. The method comprises the following steps: (1) selecting an animal and breeding conditions; (2) recovering and culturing strains; (3) partially and physically stimulating tibias; (4) anesthetizing; (5) preparing the bone carbuncle model; (6) observing and treating after an operation; (7) evaluating the bone carbuncle model; (8) applying the bone carbuncle rabbit model to evaluating the bone nonunion treating effects of the bone carbuncles. According to the bone carbuncle rabbit model developed by the invention, an animal model and detection indexes can be provided for promoting the screening and development of bone nonunion treating drugs of the bone carbuncles.

Description

technical field [0001] The invention relates to the field of experimental animals, in particular to the preparation of a carbuncle carbuncle model rabbit and the application of the model to evaluate the therapeutic effect of carbuncle carbuncle nonunion. Background technique [0002] Bone injury combined with infectious disease occurs frequently in orthopedic surgery, and gangrene due to bacterial infection is common. Bone Abscess (BA), which is called Chronic Osteomyelitis (CO) in modern medicine, is characterized by long course of disease, repeated attacks, long-term non-union, and the incidence of nonunion is as high as 10-15 %, resulting in a high disability rate. At present, surgical debridement combined with antibiotic local sustained-release system administration is used in clinical practice to achieve local anti-infection effect, and some progress has been made, reducing the recurrence rate. However, the time for bone healing is long, and bone repair and reconstruc...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61D7/00A61K35/74
CPCA61D1/00
Inventor 张扬黄孝闻沈立锋王绪平俞忠明董宇
Owner ZHEJIANG ACAD OF TRADITIONAL CHINESE MEDICINE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com